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Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification

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Title Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification
 
Creator Zhengyu Wen
Juliana, Philomin
Dhugga, Harshaant S.
Pacheco, Mario
Martínez, Ulises I.
Aguilar, Agustin
Ibba, Maria I.
Velu, Govindan
Singh, Ravi P.
Dhugga, Kanwarpal
 
Subject biofortification
grain
nutrition
iron
zinc
phytic acid
genomes
 
Description Biofortification of cereal grains offers a lasting solution to combat micronutrient deficiency in developing countries where it poses developmental risks to children. Breeding efforts thus far have been directed toward increasing the grain concentrations of iron (Fe) and zinc (Zn) ions. Phytic acid (PA) chelates these metal ions, reducing their bioavailability in the digestive tract. We present a high-throughput assay for quantification of PA and its application in screening a breeding population. After extraction in 96-well megatiter plates, PA content was determined from the phosphate released after treatment with a commercially available phytase enzyme. In a set of 330 breeding lines of wheat grown in the field over 3 years as part of a Harvest Plus breeding program for high grain Fe and Zn, our assay unraveled variation for PA that ranged from 0.90 to 1.72% with a mean of 1.24%. PA content was not associated with grain yield. High yielding lines were further screened for low molar PA/Fe and PA/Zn ratios for increased metal ion bioavailability, demonstrating the utility of our assay. Genome-wide association study revealed 21 genetic associations, six of which were consistent across years. Five of these associations mapped to chromosomes 1A, 2A, 2D, 5A, and 7D. Additivity over four of these haplotypes accounted for an ∼10% reduction in PA. Our study demonstrates it is possible to scale up assays to directly select for low grain PA in forward breeding programs.
 
Date 2022-02-15
2022-12-28T14:59:56Z
2022-12-28T14:59:56Z
 
Type Journal Article
 
Identifier Wen, Z., Juliana, P., Dhugga, H.S., Pacheco, M., Martínez, U.I., Aguilar, A., Ibba, M.I., Govindan, V., Singh, R.P. and Dhugga, K.S. 2022. Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification. Frontiers in Plant Science 13:830147. https://hdl.handle.net/10883/21954
1664-462X
https://hdl.handle.net/10568/126372
https://hdl.handle.net/10883/21954
https://doi.org/10.3389/fpls.2022.830147
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Source Frontiers in Plant Science