Record Details

Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover rates

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover rates
Not Available
 
Creator Leelavathi Sadhu
Krishan Kumar
Saravanan Kumar
Abhishek Dass
Ranjana Pathak
Amit Bhardwaj
Pankaj Pandey
Nguyen Van Cuu
Bhupendra S. Rawat
Vanga Siva Reddy
 
Subject Chloroplast
Terrestrial plant
non-AUG start codon
 
Description Not Available
Chloroplasts have evolved from photosynthetic cyanobacteria-like progenitors through endosymbiosis. The chloroplasts of present-day land plants have their own transcription and translation systems that show several similarities with prokaryotic organisms. A remarkable feature of the chloroplast translation system is the use of non-AUG start codons in the protein synthesis of certain genes that are evolutionarily conserved from Algae to angiosperms. However, the biological significance of such use of non-AUG codons is not fully understood. The present study was undertaken to unravel the significance of non-AUG start codons in vivo using the chloroplast genetic engineering approach. For this purpose, stable transplastomic tobacco plants expressing a reporter gene i.e. uidA (GUS) under four different start codons (AUG/UUG/GUG/CUG) were generated and β-glucuronidase (GUS) expression was compared. To investigate further the role of promoter sequences proximal to the start codon, uidA was expressed under two different chloroplast gene promoters psbA and psbC that use AUG and a non-AUG (GUG) start codons, respectively, and also showed significant differences in the DNA sequence surrounding the start codon. Further, to delineate the role of RNA editing that creates AUG start codon by editing non-AUG codons, if any, which is another important feature of the chloroplast transcription and translation system, transcripts were sequenced. In addition, a proteomic approach was used to identify the translation initiation site(s) of GUS and the N-terminal amino acid encoded when expressed under different non-AUG start codons. The results showed that chloroplasts use non-AUG start codons in combination with the translation initiation site as an additional layer of gene regulation to over-express proteins that are required at high levels due to their high rates of turnover.
Not Available
 
Date 2023-01-20T04:35:42Z
2023-01-20T04:35:42Z
2023-01-17
 
Type Research Paper
 
Identifier Sadhu, L., Kumar, K., Kumar, S. et al. Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover rates. Sci Rep 13, 896 (2023). https://doi.org/10.1038/s41598-022-27347-9
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/75553
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature