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Rapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa

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Title Rapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa
 
Creator Abkallo, Hussein M.
Svitek, Nicholas
Oduor, Bernard
Awino, Elias
Henson, Sonal P.
Oyola, Samuel O.
Mwalimu, Stephen
Assad-Garcia, N.
Fuchs, W.
Vashee, S.
Steinaa, Lucilla
 
Subject african swine fever virus
animal diseases
genomics
crispr
 
Description African swine fever virus (ASFV) is the etiological agent of a contagious and fatal disease of domestic pigs that has significant economic consequences for the global swine industry. Due to the lack of effective treatment and vaccines against African swine fever, there is an urgent need to leverage cutting-edge technologies and cost-effective approaches for generating and purifying recombinant virus to fast-track the development of live-attenuated ASFV vaccines. Here, we describe the use of the CRISPR/Cas9 gene editing and a cost-effective cloning system to produce recombinant ASFVs. Combining these approaches, we developed a recombinant virus lacking the non-essential gene A238L (5EL) in the highly virulent genotype IX ASFV (ASFV-Kenya-IX-1033) genome in less than 2 months as opposed to the standard homologous recombination with conventional purification techniques which takes up to 6 months on average. Our approach could therefore be a method of choice for less resourced laboratories in developing nations.
 
Date 2021-08-30
2021-08-30T13:18:57Z
2021-08-30T13:18:57Z
 
Type Journal Article
 
Identifier Abkallo, H.M., Svitek, N., Oduor, B., Awino, E., Henson, S.P., Oyola, S.O., Mwalimu, S., Assad-Garcia, N., Fuchs, W., Vashee, S. and Steinaa, L. 2021. Rapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa. Frontiers in Genetics 12: 733674.
1664-8021
https://hdl.handle.net/10568/114800
https://doi.org/10.3389/fgene.2021.733674
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 733674
 
Source Frontiers in Genetics