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Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

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Title Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
 
Creator Kante, M.
Lindqvist-Kreuze, H.
Portal, L.
David, M.
Gastelo, M.
 
Subject potatoes
phytophthora infestans
quality control
genetics
 
Description Potato virus Y (PVY) and Phytophthora infestans (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY Ryadg were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full- sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.
 
Date 2021-11-16
2021-12-16T04:36:00Z
2021-12-16T04:36:00Z
 
Type Journal Article
 
Identifier Kante, M., Lindqvist-Kreuze, H., Portal, L., David, M., & Gastelo, M. (2021). Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains. Agronomy. ISSN 2073-4395. 11(11), 2315.
2073-4395
https://hdl.handle.net/10568/116774
https://doi.org/10.3390/agronomy11112315
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 15 p.
 
Source Agronomy