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An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)

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Title An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)
 
Creator Jiménez Polo, Jenyfer
Leiva, Ana Maria
Olaya, Cristian A.
Acosta-Trujillo, Daniela
Cuéllar, Wilmer Jose
 
Subject nucleic acids
diagnostic techniques
methods
acidos nucléicos
técnicas de diagnosis
métodos
 
Description Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue.
 
Date 2021-08-24
2022-08-25T07:39:11Z
2022-08-25T07:39:11Z
 
Type Journal Article
 
Identifier Jimenez, J.; Leiva, A.M.; Olaya, C.; Acosta-Trujillo, D.; Cuellar, W. (2021) An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz). MethodsX 8: 101496. 7 p. ISSN: 2215-0161
2215-0161
https://hdl.handle.net/10568/120937
https://doi.org/10.1016/j.mex.2021.101496
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 7 p.
application/pdf
 
Publisher Elsevier
 
Source MethodsX