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Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture

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Title Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture
 
Creator Tchatchambe, N.B.J.
Ibanda, N.
Adheka, G.
Onautshu, O.
Swennen, Rony L.
Dhed’a, D.
 
Subject banana bunchy top virus
in vitro culture
tissue culture
plantains
 
Description Open Access Article
Banana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plantain Litete (Musa AAB) and the False Horn plantain Libanga Likale (Musa AAB) with advanced BBTD symptoms were collected. Meristematic apices excised from those suckers were cultured in vitro and subcultured five times. The presence of BBTV was evaluated by the Triple-Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). The BBTV was confirmed in all suckers prior to in vitro culture but 73.3% of Litete plantlets and 66.6% of Libanga Likale plantlets regenerated from meristematic tissues were virus-free. This indicates that in vitro culture is a simple tool to generate BBTV-free plantains.
 
Date 2020-03-31
2020-03-12T13:53:59Z
2020-03-12T13:53:59Z
 
Type Journal Article
 
Identifier Tchatchambe, N.B.J., Ibanda, N., Adheka, J.G., Onautshu, O.D., Swennen, R. & Dhed'a, D.B. (2020). Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture. African Journal of Agricultural Research, 15(3), 361-366.
1991-637X
https://hdl.handle.net/10568/107726
https://doi.org/10.5897/AJAR2019.14522
BIOTECH & PLANT BREEDING
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 361-366
application/pdf
 
Publisher Academic Journals
 
Source African Journal of Agricultural Research