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Marker-assisted backcross breeding for heat tolerance in bread wheat (Triticum aestivum L.)

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Title Marker-assisted backcross breeding for heat tolerance in bread wheat (Triticum aestivum L.)
Not Available
 
Creator Amasiddha Bellundagi
K.T. Ramya
Hari Krishna
Neelu Jain
P. Shashikumara
Pradeep Kumar Singh
Gyanendra Pratap Singh
Kumble Vinod Prabhu
 
Subject heat tolerance
MABB
markers
backcross
early anthesis
 
Description Not Available
Manipulation of flowering time for adaptation through natural or genetic
approaches may combat heat-stress damage that occurs at the reproductive
stages in production conditions. HD2733, a popular wheat variety of the eastern
plains of India, is largely sensitive to heat stress. Therefore, the current study
aims to improve heat tolerance of HD2733 by introgression of QTLs associated
with early anthesis and high kernel weight linked to markers Xbarc186 and
Xgwm190, respectively, through marker-assisted backcross breeding (MABB)
from a tolerant donor, WH730. A total of 124 simple sequence repeat (SSR)
markers distributed evenly across the genome were used for the background
selection. The alleles of Xbarc186 and Xgwm190 were fixed in BC2F1 and BC1F2
generations by selecting individual plants heterozygous for both marker loci
and backcrossed with HD2733 and simultaneously selfed to generate BC2F1 and
BC1F2 populations, respectively. Furthermore, the selected BC1F2 were selfed to
generate the BC1F4 population. By background screening, a total of 39 BC2F3
and 21 BC1F4 families homozygous for the targeted QTLs with 90.9–97.9% and
86.8–88.3% RPG recoveries were selected. The best performing 17 BC2F3 and
10 BC1F4 lines were evaluated for various morpho-physiological traits.
Phenotypic evaluation and multi-location trials of the introgressed lines
under late sown conditions led to the selection of three promising lines with
early anthesis and higher grain yield. The improved lines will serve as an
excellent genetic material for functional genomics and expression studies to
understand the molecular mechanisms and pathways underlying the stress
tolerance.
Not Available
 
Date 2023-01-25T08:56:00Z
2023-01-25T08:56:00Z
2022-12-01
 
Type Research Paper
 
Identifier Bellundagi A, Ramya KT, Krishna H, Jain N, Shashikumara P, Singh PK, Singh GP and Prabhu KV (2022),Markerassisted backcross breeding for heat tolerance in bread wheat (Triticum aestivum L.). Front. Genet. 13:1056783. doi: 10.3389/fgene.2022.1056783
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/75803
 
Language English
 
Relation Not Available;
 
Publisher Frontiers