Record Details

Cloning, expression and bioassay of Vip3A protein from an indigenous Bacillus thuringiensis isolate.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Cloning, expression and bioassay of Vip3A protein from an indigenous Bacillus thuringiensis isolate.
Not Available
 
Creator Rajagopal Rangeshwaran
Velavan Viswakethu
Frenita M Lewis
Surabhi Kumari
A.N. Shylesha
M. Mohan
Satendra Kumar
G. Sivakumar
 
Subject Indigenous, Bacillus thuringiensis, cloning,expression,bioassay, vip3A gene, vip3A protein
 
Description Not Available
The present study was undertaken to test vip3A protein from Bacillus thuringiensis for use against lepidopteran pests especially Spodoptera litura and whether cloning and expression of the vip3A gene will help in formulating an improved bioinsecticide. Eight isolates that showed amplification of the vip3A gene (675bp) were selected from 150 B. thuringiensis isolates. The isolate BT-EG1 was selected for further studies as the vip3A protein extracts from this isolate was found to cause mortality of Spodoptera litura (LC 50 of 9.09-9.92 µg/ml). The 2.367bp complete CDS was amplified using VCL1 and VCL2 specific primers and cloned into P UC19 at NdeI and XhoI restriction sites. The positive clones were sequence confirmed. The vip3A was the successfully cloned into pET21a (NdeI/XhoI) expression vector and confirmed by restriction digestion and SDS-PAGE. The crude protein extracts obtained after 16h of IPTG induction showed a LC 50 of 5.83 µg/ml against S. litura at 72h. The extracts were however highly toxic to Plutella xylostella (LC 50 of 0.43 µg/ml after 16h of IPTG induction at 48h). The extracts however did not cause mortality of Helicoverpa armigera suggested the requirement of a combination of vip proteins for broad spectrum activity.
Not Available
 
Date 2023-01-23T06:18:28Z
2023-01-23T06:18:28Z
2016-06-30
 
Type Journal
 
Identifier Not Available
0973-7510
http://krishi.icar.gov.in/jspui/handle/123456789/75562
 
Language English
 
Relation Not Available;
 
Publisher Not Available