Record Details

Molecular and cytogenetic characterization of wild Musa species

CGSpace

View Archive Info
 
 
Field Value
 
Title Molecular and cytogenetic characterization of wild Musa species
 
Creator Cizkova, J.
Hribova, E.
Christelova, P.
Houwe, I. van den
Hakkinnen, M.
Roux, N.
Swennen, Rony L.
Doležel, Jaroslav
 
Subject musa
morphology
dna
genotypes
microsatellites
 
Description All relevant data are within the paper and its Supporting Information files.
The production of bananas is threatened by rapid spreading of various diseases and adverse environmental conditions. The preservation and characterization of banana diversity is essential for the purposes of crop improvement. The world's largest banana germplasm collection maintained at the Bioversity International Transit Centre (ITC) in Belgium is continuously expanded by new accessions of edible cultivars and wild species. Detailed morphological and molecular characterization of the accessions is necessary for efficient management of the collection and utilization of banana diversity. In this work, nuclear DNA content and genomic distribution of 45S and 5S rDNA were examined in 21 diploid accessions recently added to ITC collection, representing both sections of the genus Musa. 2C DNA content in the section Musa ranged from 1.217 to 1.315 pg. Species belonging to section Callimusa had 2C DNA contents ranging from 1.390 to 1.772 pg. While the number of 45S rDNA loci was conserved in the section Musa, it was highly variable in Callimusa species. 5S rRNA gene clusters were found on two to eight chromosomes per diploid cell. The accessions were genotyped using a set of 19 microsatellite markers to establish their relationships with the remaining accessions held at ITC. Genetic diversity done by SSR genotyping platform was extended by phylogenetic analysis of ITS region. ITS sequence data supported the clustering obtained by SSR analysis for most of the accessions. High level of nucleotide diversity and presence of more than two types of ITS sequences in eight wild diploids pointed to their origin by hybridization of different genotypes. This study significantly expands the number of wild Musa species where nuclear genome size and genomic distribution of rDNA loci is known. SSR genotyping identified Musa species that are closely
related to the previously characterized accessions and provided data to aid in their classification. Sequence analysis of ITS region provided further information about evolutionary relationships between individual accessions and suggested that some of analyzed accessions were interspecific hybrids and/or backcross progeny.
 
Date 2015
2016-01-05T09:52:33Z
2016-01-05T09:52:33Z
 
Type Journal Article
 
Identifier Cizkova, J.; Hribova, E.; Christelova, P.; Van den houwe, I.; Hakkinnen, M.; Roux, N.; Swennen, R.; Dolezel, J. (2015) Molecular and cytogenetic characterization of wild Musa species. PLOS ONE 10(8): e0134096. ISSN: 1932-6203
1932-6203
https://hdl.handle.net/10568/69461
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134096
https://doi.org/10.1371/journal.pone.0134096
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher Public Library of Science
 
Source PLOS ONE