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Optimization of carrageenan production from Persian Gulf algae Laurencia obtusa: Purification and rheological characterization

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Title Optimization of carrageenan production from Persian Gulf algae Laurencia obtusa: Purification and rheological characterization
 
Creator Entezari, Tayebeh
Zarei, Mandana
Azari, Ahmad
 
Subject Barcoding
COI
Design-Expert
RbcL
Rheometric analysis
 
Description 565-573
In this study, Laurencia obtusa (Yamada, 1931) species was investigated for carrageenan extraction. L. obtusa species
was identified using the molecular barcoding method with two COI and rbcL genes. Rheological analysis was performed to
determine the rheological properties of semi-refined carrageenans. Experimental design was carried out with Design Expert
software to produce and optimize SRC extraction. The software presented 18 treatments based on temperature, cooking time
and concentration of KOH. Treatments were prepared for rheometric analysis (i.e., viscosity measurements). Optimization
was performed by the software with maximum viscosity. Also, two refined carrageenan extraction methods were performed,
and the FTIR and NMR spectra were compared. Barcoding showed similarity values of 96 and 94% using the COI sequence
and therbcL sequence, respectively. The optimal treatment included temperature of 77°C, 99 minutes, and 7% concentration
of w/v KOH. The highest efficiency was associated with the dialysis method, which was 19%. Based on the rheometric
analysis, the SRC solution was introduced as a pseudo plastic Non-Newtonian fluid. According to these two analyses, the
refined carrageenans of L. obtusa were of the iota type.
 
Date 2023-02-01T08:54:48Z
2023-02-01T08:54:48Z
2023-01
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61314
https://doi.org/10.56042/ijnpr.v13i4.45637
 
Language en
 
Relation A23L 29/00
A23L 29/256
A61K 31/731
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(4) [December 2022]