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Phytochemical analysis, biological activities, and GC profiling of extracts of some medicinal plant growing in Nepal

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Title Phytochemical analysis, biological activities, and GC profiling of extracts of some medicinal plant growing in Nepal
 
Creator Banjara, Rama
Sharma, Khaga Raj
Pokharel, Yuba Raj
 
Subject Antimicrobial
Antioxidant
Diabetes
DPPH
Jasminum humile
Medicinal plants
 
Description 505-515
The present study was aimed to determine the antioxidant, α amylase inhibition, and antibacterial activities on ten traditionally used medicinal plant extracts, namely Cyperus rotundus, Citrus medica, Gaultheria fragrantissima, Jasminum humile, Osyris wightiana, Buddleja asiatica, Berberis aristata, Robus ellipticus, Schima wallichii, and Smilax ovalifolia growing in Nepal. The bioactive fraction of J. humile was subjected for GC analysis. The free radical scavenging properties of plant extracts were assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and antibacterial activity was performed by the well diffusion method. The antidiabetic activity was assessed α amylase inhibition assay. The chemical compounds were isolated from the active plant fraction by silica column chromatography, and the collected fractions were analyzed by GC and FTIR. The phytochemical analysis showed that plant extracts were rich sources of secondary metabolites. The in vitro antioxidant activity showed IC50 ranging from 30.57±0.02 to 155.65±0.10 μg/mL. The promising antioxidant activity was demonstrated by S. wallichii of IC50 30.57±0.02 μg/mL and J. humile 35.28±0.54 μg/mL, respectively whereas, the S.ovalifolia, exhibited the moderate antioxidant activity of IC50 155.65±0.10 μg/mL. The J. humile showed significantantidiabetic activity of IC50 59.4±23.47 μg/mL. The antidiabetic activities exhibited ranged from IC50 of 77.29±2.05(S. wallichii to 608.28±71.50 μg/mL (C. rotundus). The R. ellipticus showed maximum ZOI (22 mm) against theStaphylococcus aureus (ATCC 25923), whereas J. humile (20 mm), O. wightiana (18 mm), and G. fragrantissima (16 mm)showed moderate antibacterial activity against the S. aureus. The C. rotundus, J. humile, S. ovalifolia, O. wightiana, andB.asiatica showed promising antibacterial activity against E. coli (ATCC 25922) with ZOI 15, 17, 14, 17, and 18 mmrespectively. These findings provide partial scientific support for traditional uses of these medicinal plants against diabetesand infectious diseases. Therefore, the J. humile could be a promising source of natural antidiabetic and antioxidantcompounds that may be drug candidates for future drug development. To the best of our knowledge, this work is the firstattempt to perform all these biological activities and phytochemical analyses growing in the particular region of Nepal.
 
Date 2023-02-01T09:33:07Z
2023-02-01T09:33:07Z
2023-02
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61321
https://doi.org/10.56042/ijnpr.v13i4.39187
 
Language en
 
Relation A61K 36/00
A61P 3/00
A61P 3/10
A61P 31/00
A61P 39/00
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(4) [December 2022]