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The effects of methanolic extract of Uncaria gambir against microflora of dental caries

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Title The effects of methanolic extract of Uncaria gambir against microflora of dental caries
 
Creator Murad, Nur Fazwani Ahmad
Mahyuddin, Alida
Shafie, Zaleha
Sockalingam, S Nagarajan MP
Zakaria, Ahmad Shuhud Irfani
 
Subject Antibacterial
Enterococcus faecalis
Lactobacillus casei
Streptococcus mutans
Streptococcus sobrinus
Uncaria gambir
 
Description 497-504
The antibacterial effects of Uncaria gambir extract have the potential to be expanded in dentistry, mainly in the management
of dental caries and its sequelae. This preliminary study was conducted to investigate the in vitro antibacterial activity of methanolic
extract of U. gambir against dental caries-related microflora: Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei
and Enterococcus faecalis. The methanolic extract of U. gambir in powder form was dissolved and serially diluted in 1% dimethyl
sulfoxide. The antibacterial effects of the extract were determined using the broth microdilution technique. A transmission electron
microscope (TEM) was used to assess the effects of the extract on the morphology of the bacteria. A 0.12% chlorhexidine (CHX)
and Man-Rogosa-Sharpe/brain heart infusion broth were used as positive and negative control respectively. Greatest antibacterial
effects were seen on both Streptococci species with the minimal inhibitory concentration (MIC) and minimal bactericidal
concentration (MBC) values of 1.25 and 5 mg/mL respectively, followed by Enterococcus faecalis (MIC=2.5 mg/mL, MBC=10
mg/mL) and Lactobacillus casei (MIC=7.5 mg/mL, MBC=30 mg/mL) in ascending order. Cell wall damage of all bacteria at their
respective MIC value was observed through the TEM analysis. Tukey’s posthoc test showed no statistically significant difference in
the antibacterial activity exerted by U. gambir extract and 0.12% CHX, with P> 0.05. Conclusively, U. gambir extract exhibits a
good antibacterial effect against the microflora of dental caries and carries great potential for future development.
 
Date 2023-02-01T09:40:00Z
2023-02-01T09:40:00Z
2023-02
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61322
https://doi.org/
 
Language en
 
Relation A61K 36/00
A61K 36/74
A61K 127/00
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(4) [December 2022]