Introgression of the low phytic acid locus (lpa2) into elite maize (Zea mays L.) inbreds through marker‑assisted backcross breeding (MABB)
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Title |
Introgression of the low phytic acid locus (lpa2) into elite maize (Zea mays L.) inbreds through marker‑assisted backcross breeding (MABB)
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Creator |
K. R. Yathish
Chikkappa Gangadhar Karjagi Shivraj Singh Gangoliya A. Kumar J. Preeti Hemant Kumar Yadav Shraddha Srivastava Santosh Kumar H. K. M. Swamy Alla Singh Ramesh Kumar Phagna Abhijit Kumar Das Javaji Chandra Sekhar Firoz Hossain Sujay Rakshit Ravindra N. Gadag |
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Subject |
Inorganic phosphorus · Maize · Marker assisted backcross breeding · Near-isogenic lines · Phytic acid
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Description |
Not Available
Phytic acid (PA) is an important antinutritional component in maize that affects the availability of major micro-nutrients like di- and multivalent mineral cations like iron (Fe) and zinc (Zn). The long-term consumption of maize as a staple food crop leads to micronutrient malnutrition especially iron and zinc deficiency in the human population. In addition, it also acts as a storehouse of a major part of mineral phosphorous (P), approximately 80% of the total P stored as phytate P is not available to monogastric animals like humans and poultry birds, and it gets excreted as such, leading to one of the major environmental pollution called eutrophication. Of the various low phytic acid (lpa) mutants, lpa2-2 generated through mutagenesis reduces PA by 30%. BML 6 and BML 45, the parents of the popular maize hybrid DHM 121 with high PA were selected to introgress lpa2-2 through marker-assisted backcross breeding (MABB). The percent recurrent parental genome (RPG) in the selected BC2F2 plants ranged from 88.68 to 91.04% and 90.09–91.51% in the genetic background of BML 6 and BML 45, respectively. Based on the highest percentage of RPG, best five BC2F2 plants, viz., #3190, #3283, #3230, #3263 and #3292 with RPG 88.68–91.04% in the genetic background of BML 6 and #3720, #3776, #3717, #3828 and #3832 with RPG 90.09–91.51% in the genetic background of BML 45 were advanced to BC2F3. The newly developed near-isogenic lines (NILs) possessed low phytate content (2.37 mg/g in BML 6 and 2.40 mg/g in BML 45) compared to 3.59 mg/g and 3.16 mg/g in recurrent parents BML 6 and BML 45, respectively thereby reducing the phytate by an average of 34 and 24 per cent, respectively. These newly developed progenies were similar to their recurrent parents for various morphological traits. These inbreds assume great significance in alleviating Fe and Zn deficiencies in worldwide. Not Available |
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Date |
2023-02-03T15:17:55Z
2023-02-03T15:17:55Z 2022-08-16 |
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Type |
Research Paper
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Identifier |
Yathish KR, Chikkappa GK, Shivraj SG, A. Kumar, J. Preeti, Hemant KY, Shraddha S, Santosh K, H. K. M. Swamy, A Singh, Ramesh KP, Abhijit K Das, JC Sekhar, F Hossain, S Rakshit and Ravindra N. Gadag (2022) Introgression of the low phytic acid locus (lpa2) into elite maize (Zea mays L.) inbreds through marker‑assisted backcross breeding (MABB). Euphytica 218:127 https://doi.org/10.1007/s10681-022-03076-y.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/75967 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer Nature
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