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Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal

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Title Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal
 
Creator Pant, Bimala
Bai, Tingting
Du, Chanjuan
Baidya, Suraj
Magar, Prem Bahadur
Manandhar, Shrinkhala
Shrestha, Jiban
Dita Rodriguez, Miguel Angel
Rouard, Mathieu
Fu, Gang
Zheng, Sijun
 
Subject bananas
fusarium oxysporum
pathogenic fungi
detection
identification
pathogens
 
Description Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies.
 
Date 2023-02-05
2023-02-07T16:43:21Z
2023-02-07T16:43:21Z
 
Type Journal Article
 
Identifier Pant, B.; Bai, T.; Du, C.; Baidya, S.; Magar, P.B.; Manandhar, S.; Shrestha, J.; Dita, M.; Rouard, M.; Fu, G.; Zheng, S.-J. (2023) Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal. Journal of Fungi 9: 208. ISSN: 2309-608X
2309-608X
https://hdl.handle.net/10568/128495
https://doi.org/10.3390/jof9020208
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher MDPI AG
 
Source Journal of Fungi