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Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU

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Title Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU
 
Creator Kumari, Safaa
Moukahel, Abdulrahman
 
Subject food security
molecular characterization
virus diseases
plant health
Faba bean
Lentil
Chickpea
Grass pea (Lathyrus sativus)
seed health testing
seed-borne virus
Field pea (Pisum sativum)
 
Description Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) is the most important virus infecting lentil both in terms of economic importance and geographical distribution. It was reported that PSbMV can even lead up to 61% yield loss. Therefore, developing and implementing effective management strategies for PSbMV is very important to reduce yield and quality loss. However, accurate detection of virus disease is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated reverse transcription-polymerase chain reaction (RT-PCR) assay for PSbMV detection and characterization applied by ICARDA’s GHU.
 
Date 2022-12-01
2023-01-24T03:04:45Z
2023-01-24T03:04:45Z
 
Type Tool
 
Identifier Safaa G. Kumari and Abdelrahman Moukahel. 2022. Molecular tool for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.
https://hdl.handle.net/10568/127945
 
Language en
 
Rights CC-BY-SA-4.0
Open Access
 
Format application/pdf
application/pdf
 
Publisher International Center for Agricultural Research in the Dry Areas