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On-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables.

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Title On-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables.
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Creator Mukherjee S, Pal S, Paria P, Bhattacharyya S, Ghosh K, Pal A, Ghosh D, Singh OK, Sarkar P, Behera BK, Das SCS, Bhand S and Bhattacharyya N
 
Subject EnzymeBiosensorOrganophosphate hydrolasePesticidesImaging arrayFruits and vegetables
 
Description Not Available
Acetylcholinesterase (AChE), a widely used enzyme for inhibition-based biosensors in pesticide residues detection, lags due to multiple-step operation, time-consuming incubation and reactivation/regeneration steps. Herein, this endeavour reports the development of Organophosphate Hydrolase (OPH), which has functional superiority over the AChE and explored in on-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables. The organophosphate degrading enzyme OPH is expressed from the ‘opd’ gene through biotechnological tools. The OPH exhibited its best activity at pH 8.0 and subsequently thermal inactivation over 37 °C. The activity of the purified OPH enzyme was found 2.75 U mL−1 at λmax 410 nm. Furthermore, the developed OPH is integrated into 96 well plate format with our previously reported UIISScan 1.1, an advanced imaging array technology based field-portable high-throughput sensory system. The developed biosensor revealed a linear range from 100 ng mL−1 to 0.1 ng mL−1 for detection of organophosphate pesticide residues with a negative slope i.e. y = 235.678x (ng mL−1) – 62.8725 with R2 = 0.99991 and n = 23. Moreover, the applicability of the developed biosensor was tested for market available fruits and vegetables. This is the first-ever reported OPH mediated on-spot biosensing device for pesticide residue detection in fruits and vegetables to the best of our knowledge.
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Date 2022-06-24T07:17:48Z
2022-06-24T07:17:48Z
2021-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/73393
 
Language English
 
Relation Not Available;
 
Publisher Not Available