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Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India

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Title Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India
Aquaculture Research
 
Creator Ramesh Chandra Malick
A.K. Bera
Hemanta Chowdhury
Manojit Bhattacharya
Tanuja Abdulla
Himanshu Sekhar Swain
Raju Baitha
Vikas Kumar
Basanta Kumar Das
 
Subject Acinetobacter junii,Acinetobacter pittii,concurrent infection,emerging pathogen,Hypophthalmichthys molitrix,Labeo catla
 
Description Not Available
The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii b y b iochemical c haracterization a nd 1 6S r RNA g ene a mplification. Both the isolates were oxidase-negative, nitrate-negative, catalase-positive and indole-negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%? 99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to ?-lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD50 of 1.24 ? 105 and 1.88 ? 107 cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug-resistant (MDR)-A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland
 
Date 2021-10-21T09:18:05Z
2021-10-21T09:18:05Z
2020-2-14
 
Type Research Paper
 
Identifier —
Not Available
1355-557X
http://krishi.icar.gov.in/jspui/handle/123456789/66072
 
Language English
 
Relation —
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Publisher Not Available