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Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation

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Title Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation
 
Creator Jaramillo, Angélica M.
Sierra, Santiago
Chavarriaga-Aguirre, Paul
Castillo, Katherine Diana
Gkanogiannis, Anestis
Becerra Lopez-Lavalle, Luis Augusto
Arciniegas, Juan Pablo
Sun, Tianhu
Li, Li
Welsch, Ralf
Boy, Erick
Álvarez, Daniel
 
Subject provitamins
carotenoids
protein requirements
provitaminas
carotenoides
requerimientos proteicos
 
Description Cassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to increase carotenoid content focused on genes from the carotenoids biosynthesis pathway. In recent years, special emphasis was given to ORANGE protein (OR), which promotes the accumulation of carotenoids and their stability in several plants. The aim of this work was to identify, characterize and investigate the role of OR in the biosynthesis and stabilization of carotenoids in cassava and its relationship with phytoene synthase (PSY), the rate-limiting enzyme of the carotenoids biosynthesis pathway. Gene and protein characterization of OR, expression levels, protein amounts and carotenoids levels were evaluated in roots of one white (60444) and two yellow cassava cultivars (GM5309-57 and GM3736-37). Four OR variants were found in yellow cassava roots. Although comparable expression was found for three variants, significantly higher OR protein amounts were observed in the yellow varieties. In contrast, cassava PSY1 expression was significantly higher in the yellow cultivars, but PSY protein amount did not vary. Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. Co-expression with MeOR_X1 led to greatly increase of carotenoids although PSY1 expression was high in the co-expressed FEC. Our data suggest that posttranslational mechanisms controlling OR and PSY protein stability contribute to higher carotenoid levels in yellow cassava. Moreover, we showed that cassava FEC can be used to study the efficiency of single and combinatorial gene expression in increasing the carotenoid content prior to its application for the generation of biofortified cassava with enhanced carotenoids levels.
 
Date 2022-01-07
2023-02-09T14:44:36Z
2023-02-09T14:44:36Z
 
Type Journal Article
 
Identifier Jaramillo, A.M.; Sierra, S.; Chavarriaga-Aguirre, P., Castillo, K.D.; Gkanogiannis, A.; Becerra López-Lavalle, L.A.; Arciniegas, J.P.; Sun, T.; Li, L.; Welsch, R.; Boy, E.; Álvarez, D. (2022) Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation. PLoS One 17(1): e0263168 24 p. ISSN: 1932-6203
1932-6203
https://hdl.handle.net/10568/128585
https://doi.org/10.1371/journal.pone.0263168
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 24 p.
application/pdf
 
Publisher Public Library of Science (PLoS)
 
Source PLoS ONE