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Seogroup distribution and virulence characteristics of sorbitol-negative Escherichia coli for food and cattle stool

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Title Seogroup distribution and virulence characteristics of sorbitol-negative Escherichia coli for food and cattle stool
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Creator Manna, S.K.,
Manna, C.,
Batabyal, K.,
Das, B.,
Golder, D.,
Chattopadhyay, S.
and Biswas, B. K.
 
Subject serogroup
cattle stool
 
Description Not Available
Aims: To (i) study the serogroup distribution and virulence characteristics of non-sorbitol-fermenting Escherichia coli isolates from foods of animal origin and cattle faeces and (ii) re-examine the true sorbitol and beta-D-glucuronidase (GUD) reactions of sorbitol-negative (Sor(-)) strains from MacConkey sorbitol agar (SMAC) to assess their phenotypic similarity with E. coli O157.

Methods and results: One hundred and thirty Sor(-)E. coli were isolated from 556 food samples and 177 cattle stool samples using cefixime tellurite-supplemented SMAC (CT-SMAC) and chromogenic HiCrome MS.O157 agar respectively. Based on typing of somatic antigen, the isolates were classified into 38 serogroups. PCR results identified about 40% strains, belonging to O5, O8, O20, O28, O48, O60, O78, O82, O84, O101, O110, O123, O132, O156, O157, O-rough and OUT as Shiga toxigenic. Majority of O5, O84, O101, O105, O123, O157, O-rough and OUT strains were enterohaemolytic. Further, 39.2% and 63.1% of Sor(-) isolates from CT-SMAC fermented sorbitol in phenol red broth and hydrolysed 4-methylumbelliferyl-beta-D-glucuronide (MUG) respectively. Members of serogroups O5, O28, O32, O81, O82, O84, O101, O-rough lacked both the sorbitol fermentation (broth test) and GUD activity and might create confusion in phenotypic identification of E. coli O157.

Conclusions: Sor(-)E. coli isolates from raw meat, milk, shrimp and cattle stool belonged to 38 serogroups, with E. coli O157 constituting only 14.6% of the isolates. Many of these nonclinical Sor(-) strains were potentially pathogenic. Nearly 39% of these Sor(-)E. coli from CT-SMAC fermented sorbitol in broth, indicating the need for confirmation of sorbitol reaction in broth.
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Date 2021-07-29T04:41:02Z
2021-07-29T04:41:02Z
2010-01-01
 
Type Research Paper
 
Identifier Manna, S.K., Manna, C., Batabyal, K., Das, B., Golder, D., Chattopadhyay, S. and Biswas, B. K. 2010. Seogroup distribution and virulence characteristics of sorbitol-negative Escherichia coli for food and cattle stool. Journal of Applied Microbiology. 108:658-665
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http://krishi.icar.gov.in/jspui/handle/123456789/50930
 
Language English
 
Relation Not Available;
 
Publisher Not Available