ICAR Research Data Repository for Knowledge Management
Isolation of total DNA
CMFRI Repository
View Archive Info| Field | Value | |
| Relation |
http://eprints.cmfri.org.in/8420/
|
|
| Title |
Isolation of total DNA
|
|
| Creator |
Mohindra, Vindhya
Gopalakrishnan, A Raja Swaminathan, T Paulton, M P Sajeela, K A |
|
| Subject |
Fish Genetics
|
|
| Description |
Total DNA can be extracted from fresh, frozen or fixed blood, fresh/frozen liver, muscle, sperms, kidney and any other tissue of fish. A slight modification of extraction protocol has to be done for each type as well as state of storage of tissue. Most frequently used method of DNA isolation is the phenol-chloroform method, which removes proteins and other cellular components from nucleic acids and relatively pure DNA can be obtained for further analysis.
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Identifier |
http://eprints.cmfri.org.in/8420/1/Vindhya.pdf
Mohindra, Vindhya and Gopalakrishnan, A and Raja Swaminathan, T and Paulton, M P and Sajeela, K A (2009) Isolation of total DNA. Training Manual on Advances in DNA Marker Techniques. pp. 32-45. |
|