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Evaluation of housekeeping genes as references for quantitative real-time PCR analysis of gene expression in the

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Title Evaluation of housekeeping genes as references for quantitative real-time PCR analysis of gene expression in the
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Creator Purohit GK,
Mahanty A,
Mohanty BP,
Mohanty S
 
Subject Reference genes
Quantitative real-time
PCR Normalization
Channa striatus
Thermal stress
 
Description Not Available
Quantitative real-time polymerase chain reaction is the most advanced method of quantifying gene expression studies; however, the significance of the obtained results strongly depends on the normalization of the data to compensate for differences between the samples. In the present study, expression analysis of six different constitutively expressed genes viz. 18S ribosomal RNA, glyceraldehyde-3-phosphate dehydrogenase (gapdh), beta actin (βactin), ribosomal binding protein L13, tubulin and TATA-box-binding protein (tbp) were carried out to test their efficacy as reference genes in three different tissues, namely liver, gill and muscle of murrel Channa striatus exposed to high temperature for variable time periods. The stability and suitability of the genes were determined by using bioinformatic tools: GeNorm, NormFinder and BestKeeper. Based on the results, tub/βactin could be used as the reference genes for liver and gill tissues and βactin/gapdh could be the reference genes for muscle tissues in Channa striatus under both short- and long-term thermal stress.
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Date 2019-09-11T15:16:11Z
2019-09-11T15:16:11Z
2015-01-01
 
Type Research Paper
 
Identifier Purohit GK, Mahanty A, Mohanty BP, Mohanty S (2015). Evaluation of housekeeping genes as references for quantitative real-time PCR analysis of gene expression in the murrelChannastriatus under high-temperature stress. Fish Physiology and Biochemistry 42:125-135.
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http://krishi.icar.gov.in/jspui/handle/123456789/22986
 
Language English
 
Relation Not Available;
 
Publisher Not Available