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A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment

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Title A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment
Not Available
 
Creator Priyadarshi, H.
Alam, A.,
Gireesh-Babu,
P., Das,
R., Kishore,
Kumar, S.
Chaudhari, A
 
Subject attP/attB
integrationE.
coli biosensorGFPmercury
 
Description Not Available
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of γ phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ⩽ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples.
Not Available
 
Date 2019-09-25T16:21:08Z
2019-09-25T16:21:08Z
2012-05-01
 
Type Research Paper
 
Identifier Priyadarshi, H., Alam, A., Gireesh-Babu, P., Das, R., Kishore, P., Kumar, S. and Chaudhari, A. 2012. A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment. Journal of Environmental Sciences, 24(5): 963–968.
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http://krishi.icar.gov.in/jspui/handle/123456789/23098
 
Language English
 
Relation Not Available;
 
Publisher Not Available