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Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India

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Title Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India
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Creator Ramesh Chandra Malick
Asit Kumar Bera
Hemanta Chowdhury
Manojit Bhattacharya
Tanuja Abdulla
Himanshu Sekhar Swain
Raju Baitha
Vikas Kumar
Basanta Kumar Das
 
Subject Acinetobacter junii, Acinetobacter pittii, concurrent infection, emerging pathogen, Hypophthalmichthys molitrix, Labeo catla
 
Description Not Available
The disease outbreaks in aquaculture system of wetlands are the major cause of
fish mortality. Among various bacterial septicaemic diseases, fish mortality caused
by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak
was investigated in a wetland of West Bengal, India to identify the aetiological
factors involved. The moribund fish were examined and subjected to bacterial
isolation. Two bacterial causative agents were identified as Acinetobacter junii and
Acinetobacter pittii b y b iochemical c haracterization a nd 1 6S r RNA g ene a mplification.
Both the isolates were oxidase-negative, nitrate-negative, catalase-positive and
indole-negative. The molecular identification using 16S rRNA gene sequencing and
phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–
99% similarity. The antibiotic resistance patterns of these two bacteria revealed that
both of them were resistant to β-lactam, cefalexin, cephalothin, amoxyclav, cefuroxime,
cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also
resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime.
In the challenge experiment, both A. junii and A. pittii were found to be pathogenic
with LD50 of 1.24 × 105 and 1.88 × 107 cfu/fish respectively. Histopathological examination
of gill, liver and kidney revealed prominent changes supporting bacterial
septicaemia. The investigation reports for the first time on concurrent infection by A.
junii and multidrug-resistant (MDR)-A. pittii as emerging fish pathogens to cause severe
mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland
Not Available
 
Date 2021-08-13T04:47:55Z
2021-08-13T04:47:55Z
2020-02-14
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/56206
 
Language English
 
Relation Not Available;
 
Publisher Wiley