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Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India.

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Title Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India.
Not Available
 
Creator Mallick RC
Bera AK
Chowdhury H
Bhattacharya M
Abdulla T
Swain HS
Das BK
 
Subject Acinetobacter junii Acinetobacter pittii Labeo catla Hypophthalmichthys molitrix
 
Description Not Available
The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase-negative, nitrate-negative, catalase-positive and indole-negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β-lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD50 of 1.24 × 105 and 1.88 × 107 cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug-resistant (MDR)-A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland.
Not Available
2021-08-20T01:47:26Z
2021-08-20T01:47:26Z
2020-03-09
Research Paper
Mallick RC, Bera AK, Chowdhury H, Bhattacharya M, Abdulla T, Swain HS and Das BK (2020). Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal, India. Aquaculture Research, 51(6): 2410-2420.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/58899
English
Not Available;
Aquaculture Research

oai:krishi.icar.gov.in:123456789/589002023-02-20T09:19:18Zcom_123456789_87com_123456789_7col_123456789_452
Protective effects of selected solvent extracts of Terminalia arjuna against environment mediated parasitic infection in Labeo rohita.
Not Available
Meena DK
Sahoo AK
Srivastava PP
Sahu NP
Behera BK,
Borah S,
Swain HS
Das BK
Functional screening TI &LC50 RPS & AE Argulus bengalensis Labeo rohita Solvent extracts of Terminalia arjuna
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In the present study, solvent extracts were used at 10, 20, 30, 40, and 50 mg/L, at 1, 2, 3, 4 and 5 hours and 12, 24, 36,48 and 60 hours under in-vitro and in-vivo conditions, respectively 25-30, (A. bengalensis/fish) Labeo rohita (30±1.5 g). The 5 % dimethyl sulphoxide was used as negative control (DMSO). The LC50 values of solvent extracts for L. rohita were 67.67±12.59, 78.13±14.17, 79.12±17.68, 156.47±12.67 and 256.43±8.93 mg/L for Terminalia arjuna ethanolic bark extract, Terminalia arjuna methanolic bark extract and Terminalia arjuna acetone bark extract, respectively, at 60 hours interval. Under in-vitro condition, 100 % anti-parasitic efficacy (AE) and minimum therapeutic index (TI) value (1.2) was ascertained by Terminalia arjuna ethanolic bark extract at 50 mg/L in 2 hour, and minimum LC50 was reported by Terminalia arjuna ethanolic bark extract under in-vitro condition (13.14 ±3.79 mg/L) and maximum by Terminalia arjuna acetone bark extract under in-vitro condition (75.8±12.69 mg/L) at 5 hour interval. While, under in-vivo conditions, minimum LC50 for immersion and bath treatments was observed with Terminalia arjuna ethanolic bark extract (27.92±9.56 mg/L) and TAEBIM (33.6±7.58 mg/L), correspondingly, at 60 hours. The minimum TI was reported in bath treatment of Terminalia arjuna ethanolic bark extract (1.1). The 100% anti-parasitic activity was observed in bath treatment of Terminalia arjuna ethanolic bark extract at 24 hours. The PCA bi-plot explains 79.34 % and 14.32 % variations for component 1 & 2, respectively. The efficacy of solvent extracts varied significantly in response to concentrations of the extracts and exposure times and toxicity of the extracts (Exposure time*extract *treatment: F=16.12, P=0.04). The study provides, the evidences for safe and effective application of prospective solvent extracts of T. arjuna against A. bengalensis in L. rohita juveniles, and yield first-hand information on acute toxicity of solvent extract in L. rohita.
Not Available
2021-08-20T01:47:39Z
2021-08-20T01:47:39Z
2020-01-01
Research Paper
Meena DK, Sahoo AK, Srivastava PP, Sahu NP, Behera BK, Borah S, Swain HS and Das BK (2020). Protective effects of selected solvent extracts of Terminalia arjuna against environment mediated parasitic infection in Labeo rohita. International Aquatic Research, 12(4): 267-278. doi: 10.22034/IAR.2020.1905292.1073.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/58900
English
Not Available;
International Aquatic Research

oai:krishi.icar.gov.in:123456789/589012023-02-20T09:17:49Zcom_123456789_87com_123456789_7col_123456789_452
Effect of dietary supplementation of mannan oligosaccharide on growth and survival of giant freshwater prawn, Macrobrachium rosenbergii.
Not Available
Meshram SJ
Dhamagaye HB
Swain HS
Murthy HS
Ali A
Gitte MJ
Sadawarte RK
Mannan oligosaccharide, Macrobrachium rosenbergii, growth
Not Available
Experiment was conducted in triplicate groups on freshwater prawn, Macrobrachium rosenbergii juvenile
(0.84±0.01g) to evaluate the effect of dietary supplementation of mannan oligosaccharide on growth and survival. The different
dietary mannan oligosaccharide levels were used 0.0, 1.5, 3.0, 4.5 g kg-1 diet.
After 60 days of feeding there was significant (p < 0.05) increment in terms of weight, weight gain, specific growth, survival
percentage and lower FCR recorded in 1.5 g kg-1 mannan oligosaccharide supplemented group as compared to other groups.
The results imply the positive effect of dietary supplementation of mannan oligosaccharide (1.5 g kg-1) in the culture of
freshwater prawn, Macrobrachium rosenbergii.
Not Available
 
Date 2021-08-20T01:47:52Z
2021-08-20T01:47:52Z
2020-01-01
 
Type Research Paper
 
Identifier Meshram, S. J., Dhamagaye, H. B., Swain, H. S., Murthy, H. S., Ali, A., Gitte, M. J., & Sadawarte, R. K. (2020). EFFECT OF DIETARY SUPPLEMENTATION OF MANNAN OLIGOSACCHARIDE ON GROWTH AND SURVIVAL OF GIANT FRESHWATER PRAWN, MACROBRACHIUM ROSENBERGII. Journal of Experimental Zoology, India, 23(Suppl. 1), 869-871.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/58901
 
Language English
 
Relation Not Available;
 
Publisher Journal of Experimental Zoology, India