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Differential gene expression analysis in germinating and dormant teliospores of <em>Tilletia indica</em> using RNA seq approach

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Title Statement Differential gene expression analysis in germinating and dormant teliospores of <em>Tilletia indica</em> using RNA seq approach
 
Added Entry - Uncontrolled Name M, Haritha Mohan; Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
Gurjar, Malkhan Singh; Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
Jain, Prachi ; Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
Kumar, Naresh ; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
Saharan, Mahender Singh; Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
Aggarwal, Rashmi ; Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 India
ICAR-CRP genomics (ICAR-G/CRP Genomics/ 2015-2720/IARI-12-151)
 
Uncontrolled Index Term Coding sequence; Gene ontology; Karnal bunt; Transcriptome
 
Summary, etc. Karnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market. The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession: PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91 were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to devise better management strategies to manage Karnal bunt disease.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2023-02-24 12:56:58
 
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http://op.niscair.res.in/index.php/IJEB/article/view/58626
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 61, ##issue.no## 03 (2023): IJEB [March 2023]
 
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