Record Details

Prognostic significance of microRNA-20a-5p levels which promotes proliferation and invasion by targeting cyclin G2 in small cell lung cancer

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Prognostic significance of microRNA-20a-5p levels which promotes proliferation and invasion by targeting cyclin G2 in small cell lung cancer
 
Added Entry - Uncontrolled Name Tan, Xiao-Gang
Teng, Lianghong
Wang, Wei
Gao, Wei
Zhang, Yi
 
Uncontrolled Index Term Cancer suppressor; Luciferase reporter gene assay; Tumor promoter factor; Tumorigenicity
 
Summary, etc. MicroRNA-20a-5p (miR-20a-5p) has been shown to function as a tumor promoter factor in several cancers. However, its role in small cell lung cancer (SCLC) remains unclear. In this study, we have made an attempt to measure the tumor tissue levels of miR-20a-5p in patients with SCLC using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The biological function of miR-20a-5p in SCLC cells was investigated in vitro and in vivo studies, including cell proliferation, migration assays and tumorigenicity in nude mice. Meanwhile，we conducted the luciferase reporter assay to verify the biological relationship between miR-20a-5p and CCNG2. The expression of miR-20a-5p was significantly upregulated in human SCLC compared to that in normal tissues. Kaplan-Meier analysis indicated that patients with high expression of miR-20a-5p are closely related with the shorter survival of SCLC. Further, multivariate analysis showed that miR-20a-5p was an independent prognostic factor. Increasing miR-20a-5p expression promotes the proliferation, migration and invasion of the NCI-H446 cells in vitro and in vivo. Dual-luciferase reporter gene assay demonstrated that miR-20a-5p directly targets CCNG2. These findings suggest that miR-20a-5p levels might be a novel diagnostic and prognostic marker of SCLC. Inhibiting miR-20a-5p could be a promising therapeutic strategy for SCLC.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2023-02-24 12:56:58
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/59806
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 61, ##issue.no## 03 (2023): IJEB [March 2023]
 
Language Note en