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Effect of combination treatment of protocatechuic acid with 5-fluorouracil and oxaliplatin on colon cancer Caco-2 cell line

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Title Statement Effect of combination treatment of protocatechuic acid with 5-fluorouracil and oxaliplatin on colon cancer Caco-2 cell line
 
Added Entry - Uncontrolled Name Yıldız, Fatma ; Department of Medical Laboratory Techniques, Vocational School of Health Services, Alanya Alaaddin Keykubat University, Antalya, Turkey
Eciroglu, Hamiyet ; Department of Medical Laboratory Techniques, Vocational School of Health Services, Alanya Alaaddin Keykubat University, Antalya, Turkey
Ovey, İshak Suat ; Department of Physiology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, Turkey
Avnioglu, Seda ; Department of Anatomy, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, Turkey
This research was supported by the Scientific Research Projects of Alanya Alaaddin Keykubat University (Project number 2019-15-01-MAP01).
 
Uncontrolled Index Term Anticancer activity; Caco-2; Combination index; Dose reduction index (DRI); Matrix metalloproteinases (MMP)
 
Summary, etc. Among the most common antitumor drugs used in the treatment of colon cancer are 5-fluorouracil and oxaliplatin (5-FU and OXA). However, both these drugs have many side effects, and hence there is a need for new treatment\approach to reduce the side effects aas well as drug concentration. In this context, here, we investigated the effect of addition of protocatechuic acid (PCA) onto either monotherapies or combination therapies of 5-FU and OXA on the human colon cancer (Caco-2) cell line. In addition, we did evaluate the synergistic effect of PCA with 5-FU and OXA. Further, we determined the suppressive effects of different doses of PCA alone or in combination with 5-FU/OXA on cell proliferation after 24 and 48 hours. We identified a suppressive effect of PCA on cell viability at 48 h starting from the dose of 50 µM Matrix metalloproteinase-2 (MMP-2) and MMP-9 gene expression levels and apoptotic effects showed significant increases and decreases depending on the dose and time applied in the experimental groups. The highest synergistic activity was seen at 2:1 concentration of 5-FU+ PCA. Our findings indicate the presence of the cytotoxic and apoptotic effects of PCA in Caco-2 cells at 48 h, increasing with a dose- and time-dependent manner.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2023-02-24 12:56:58
 
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http://op.niscair.res.in/index.php/IJEB/article/view/65610
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 61, ##issue.no## 03 (2023): IJEB [March 2023]
 
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