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Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach

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Title Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach
 
Creator M, Haritha Mohan
Gurjar, Malkhan Singh
Jain, Prachi
Kumar, Naresh
Saharan, Mahender Singh
Aggarwal, Rashmi
 
Subject Coding sequence
Gene ontology
Karnal bunt
Transcriptome
 
Description 214-223
Karnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market.
The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq
approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing
by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession:
PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The
high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between
dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91
were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in
germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in
number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential
expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS
were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated
using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and
Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these
differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to
devise better management strategies to manage Karnal bunt disease.
 
Date 2023-02-27T08:16:44Z
2023-02-27T08:16:44Z
2023-02
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61419
https://doi.org/10.56042/ijeb.v61i03.58626
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(03) [Mar 2023]