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Effects of culture condition and nutrition on the co-production of microbial oil and exopolysaccharide by Sporidiobolus pararoseus JD-2

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Title Effects of culture condition and nutrition on the co-production of microbial oil and exopolysaccharide by Sporidiobolus pararoseus JD-2
 
Creator Guo, Yan-Feng
Wang, Qi-Yang
Wang, Hong-Tao
Meng, Guo-Qing
Xu, Jian-Zhong
 
Subject Culture condition
Culture nutrition
Dissolve oxygen
Yeast
 
Description 196-203
Microbial oil has been gaining considerable attention from researchers recently as renewable and ecofriendly oil and its
potential as feedstock for food industry and biodiesel industry. In this context, we have earlier demonstrated production of
microbial oil and exopolysaccharide (EPS) from the yeast Sporidiobolus pararoseus JD-2. In this study, we explored
increasing its production by optimizing the culture condition and nutrition. As expected, culture temperature and dissolved
oxygen (DO) are the contributing factors for co-producing microbial oil and EPS, in which 28℃ and lower quantum (i.e., 30
mL/500 mL) show the best conditions in shake-flasks fermentation. By contrast, the initial pH from 4 to 8 has no obvious
effect on producing microbial oil and EPS. In addition, the culture nutrition (i.e., carbon/nitrogen source) were also
discussed, and indicating that 20 g/L of corn steep liquor and 60 g/L of glucose are beneficial to produce microbial oil and
EPS (i.e., 34.1±1.2 g/L and 11.5±0.2 g/L, respectively). Meanwhile, the residue glucose should be maintained at 20 g/L, in
which the highest production of microbial oil and EPS was obtained (i.e., 34.6±1.7 g/L and 11.7±0.8 g/L, respectively). The
biomass, microbial oil and EPS were further increased during optimizing the DO level, which reached to 67.8±2.1 g/L,
34.7±0.6 g/L and 11.8±0.5 g/L during maintaining DO level at 20-30%, respectively. The results suggest that appropriate
culture condition and nutrition considerably improve the fermentation performance of S. pararoseus JD-2 and significantly
increase co-production of microbial oil and EPS (by 11.2 and 8.3%, respectively) compared to the un-optimized
fermentation.
 
Date 2023-02-27T08:26:14Z
2023-02-27T08:26:14Z
2023-02
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61421
https://doi.org/10.56042/ijeb.v61i03.69807
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(03) [Mar 2023]