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Berberine derivative WJCPR11 enhances osteoblast differentiation

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Field Value
 
Title Berberine derivative WJCPR11 enhances osteoblast differentiation
 
Creator Lee, Ye Jin
Nam, Seo Woo
Park, Won-Jong
Han, Youn Ho
 
Subject Alkaline phosphatase
Bone morphogenetic protein 2
Bone sialoprotein
Osterix
Osteoporosis
Runx2
 
Description 167-174
Berberine is a phytocompound from plants viz. Phellodendri cortex and Coptis rhizome, used to treat a variety of diseases. It is effective in preventing osteoporosis, but it is less effective than drugs currently used in clinical practice. In this study, we used a novel berberine derivative, WJCPR11, to promote osteoblast differentiation and to investigate its use in the prevention and treatment of osteoporosis. WJCPR11 at a safe concentration without toxicity increased alkaline phosphatase (ALP) activity induced by bone morphogenetic protein 2 (BMP2) dose-dependently. The mRNA expression of ALP, osteocalcin (OC), runt-related transcription factor 2 (Runx2), and osterix was increased, with the ALP level increasing the most. In addition, the protein abundance of bone sialoprotein (BSP), collagen, type I, alpha 1, Runx2, and osterix were also increased. Moreover, the transcriptional activity of ALP, BSP, and OC was increased by WJCPR11, with OC showing the most significant increase. The results indicate that osteoblast differentiation is promoted by WJCPR11, and it could play a role in the prevention of osteoporosis.
 
Date 2023-02-27T08:45:49Z
2023-02-27T08:45:49Z
2023-02
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61424
https://doi.org/10.56042/ijeb.v61i03.60701
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(03) [Mar 2023]