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Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS)

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Title Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS)
Not Available
 
Creator Guru-Pirasanna-Pandi G
Soumya Bharti Babu
Jaipal Singh Choudhary
Muhammad Asad
Parameswaran Chidambaranathan
Basana-Gowda Gadratagi
Prakash Chandra Rath
Naiyar Naaz
Mariusz Jaremko
Kamal Ahmad Qureshi
Uttam Kumar 6
 
Subject phylogeny
phloem feeder
sucking pest
mitochondria
NGS
 
Description Not Available
Nilaparvata lugens is the main rice pest in India. Until now, the Indian N. lugens mito chondrial genome has not been sequenced, which is a very important basis for population genetics
and phylogenetic evolution studies. An attempt was made to sequence two examples of the whole
mitochondrial genome of N. lugens biotype 4 from the Indian population for the first time. The
mitogenomes of N. lugens are 16,072 and 16,081 bp long with 77.50% and 77.45% A + T contents,
respectively, for both of the samples. The mitochondrial genome of N. lugens contains 37 genes,
including 13 protein-coding genes (PCGs) (cox1-3, atp6, atp8, nad1-6, nad4l, and cob), 22 transfer
RNA genes, and two ribosomal RNA (rrnS and rrnL) subunits genes, which are typical of metazoan
mitogenomes. However, both samples of N. lugens mitogenome in the present study retained one
extra copy of the trnC gene. Additionally, we also found 93 bp lengths for the atp8 gene in both of
the samples, which were 60–70 bp less than that of the other sequenced mitogenomes of hemipteran
insects. The phylogenetic analysis of the 19 delphacids mitogenome dataset yielded two identical
topologies when rooted with Ugyops sp. in one clade, and the remaining species formed another
clade with P. maidis and M. muiri being sisters to the remaining species. Further, the genus Nilaparvata
formed a separate subclade with the other genera (Sogatella, Laodelphax, Changeondelphax, and Unkan odes) of Delphacidae. Additionally, the relationship among the biotypes of N. lugens was recovered as
the present study samples (biotype-4) were separated from the three biotypes reported earlier. The
present study provides the reference mitogenome for N. lugens biotype 4 that may be utilized for
biotype differentiation and molecular-aspect-based future studies of N. lugens.
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Date 2023-03-06T11:25:04Z
2023-03-06T11:25:04Z
2022-08-23
 
Type Research Paper
 
Identifier Govindharaj, G.-P.-P.; Babu, S.B.; Choudhary, J.S.; Asad, M.; Chidambaranathan, P.; Gadratagi, B.-G.; Rath, P.C.; Naaz, N.; Jaremko, M.; Qureshi, K.A.; et al. Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS). Life 2022, 12, 1289. https://doi.org/ 10.3390/life12091289
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http://krishi.icar.gov.in/jspui/handle/123456789/76555
 
Language English
 
Relation Not Available;
 
Publisher MDPI