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Stability indicating HPLC method for the simultaneous analysis of Gatifloxacin and Loteprednol in eyedrop formulation using design of experiment approach

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Title Stability indicating HPLC method for the simultaneous analysis of Gatifloxacin and Loteprednol in eyedrop formulation using design of experiment approach
 
Creator Dewani, Anil P
Vekariya, Hitesh J
Khan, Farhan R
Omar, Bashir Ibrahim A
Binshaya, Abdulkarim S
Alhazmi, Abdulfattah Yahya M
Hasan, Mohammad Raghibul
Alotaibi, Bader Saud
Chandewar, Anil V
 
Subject Central composite design
Gatifloxacin
HPLC
Loteprednol
 
Description 187-197
Design of experiment (DOE) assisted simple, rapid, precise and accurate stability indicating HPLC method has been
developed for simultaneous estimation of Gatifloxacin (GTF) and Loteprednol (LOT) along with their forced degradation
products. The developed method has been optimized and developed by using central composite design (CCD) in response
surface methodology (RSM). Trails have been undertaken and ratio of phosphate buffer in mobile phase, pH of buffer and
flow rate are selected as factors. Resolution, tailing factor (GTF) and tailing factor (LOTE) are selected for determining the
system response in the process of method optimization. The responses have been optimized using the Derringer’s
desirability function. The effective separation is achieved on Phenomenex EVO-C18 column (250 mm x 4.6 mm i.d, 5 μm
particle size) with mobile composed of 10 mM phosphate buffer, pH 3.5 and organic phase composed of mixture of
acetonitrile and methanol 60:40 % v/v, the flow rate was 1.0 mL/min, the signals were detected at 267 nm. The developed
method was validated for linearity, accuracy, precision, and robustness. The method was applied successfully for
stability samples.
 
Date 2023-03-17T10:13:31Z
2023-03-17T10:13:31Z
2023-03
 
Type Article
 
Identifier 0975-0991 (Online); 0971-457X (Print)
http://nopr.niscpr.res.in/handle/123456789/61560
https://doi.org/10.56042/ijct.v30i2.66233
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJCT Vol.30(2) [March 2023]