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In vitro mass multiplication of gerbera (Gerbera jamesonii) using capitulum explant

Indian Agricultural Research Journals

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Title In vitro mass multiplication of gerbera (Gerbera jamesonii) using capitulum explant
 
Creator Bhatia, R
Singh, K P
Singh, M C
 
Subject Capitulum; Gerbera; Growth hormones; Micropropagation
 
Description An experiment was conducted to develop highly efficient and economically viable protocol for rapid in vitro multiplication of gerbera cv. cabana using capitulum as explant during 2005–07. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. The type and concentration of cytokinin also showed profound effect on proliferation. Better proliferation of quality shoots was obtained on BAP supplemented medium than the medium containing kinetin. The maximum numbers of quality shoots were obtained on MS medium containing 1 mg/l BAP and 0.1 mg/l IAA. Half strength MS medium containing 1 mg/l IBA was optimum for root induction. This protocol can produce 85000– 430312 healthy, true-to-the-type plants per year from a single capitulum. Hence, the above protocol can be of high economic interest to the growers opting tissue culture as commercial venture.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2012-09-17
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/23830
 
Source The Indian Journal of Agricultural Sciences; Vol 82, No 9 (2012)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/23830/11374
 
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