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Probiotic treatment with Kefir reduces vascular oxidative stress while suppressing COX2 mediated relaxation in intestinal arteries of an animal model of menopause

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Title Probiotic treatment with Kefir reduces vascular oxidative stress while suppressing COX2 mediated relaxation in intestinal arteries of an animal model of menopause
 
Creator Couto, Mariana dos Reis
Oliveira, Phablo Wendell Costalonga
Januário de Sousa, Glauciene
Peixoto, Pollyana
Santos, Roger Lyrio dos
Brasil, Girlandia Alexandre
Uggere de Andrade, Tadeu
Bissoli, Nazaré Souza
 
Subject COX2
Kefir
Menopause
Mesenteric vessels
Oxidative stress
Reactive oxygen species
 
Description 284-289
Functional foods such as probiotics are known to have benefits in various diseases including metabolic disorders and
cardiovascular disease (CVD). In women, CVD has been shown to be linked with their gut microbiota and hormones. Here,
we have evaluated the effects of chronic Kefir, a fermented milk beverage in Russia, Central Asia, Middle East and Eastern
Europe, on mesenteric artery, using an animal model of menopause focusing on the superoxide anion and COX2 pathways.
Two-month-old female Wistar rats were ovariectomized and treated by gavage with Kefir (5 % w/v, 3 mL/kg/day) or milk
(Control) during two months. After this period, third-order mesenteric artery segments were isolated and mounted in a
myograph system for evaluation of concentration-response curves to acetylcholine. We performed western blot analyses and
measured oxidative stress through dihydroethidium (DHE) staining. Kefir reduced vascular oxidative stress, despite not
changing SOD2 levels. COX2 levels were not changed by kefir, despite an apparent tendency towards reduction. However,
in the functional experiments, under incubation with a COX2 inhibitor, a suppression of this pathway was observed in the
kefir group, which suggests an interaction between inflammatory pathways and oxidative stress in this model. The effect of
acute incubation with a superoxide anion scavenger on vascular responsiveness was equal in both groups. Kefir reduces
vascular oxidative stress levels while suppressing COX2-mediated relaxation in mesenteric vessels in an animal model of
menopause; which appears to involve an interplay between these two factors.
 
Date 2023-04-03T09:38:20Z
2023-04-03T09:38:20Z
2023-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61644
https://doi.org/10.56042/ijeb.v61i04.176
 
Language en
 
Source IJEB Vol.61(04) [Apr 2023]