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Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicity

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Title Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicity
 
Creator Li, Haiyan
Wang, Tongling
Liu, Dandan
Ding, Yusong
Liu, Jinbao
 
Subject Diabetic nephropathy
Iron
NF-E2-Related Factor 2
Oxidative stress
 
Description 265-275
Diabetic nephropathy (DN) is the most common cause of end-stage renal failure, oxidative damage and iron metabolism
disorder are related to DN progression, but the exact pathogenesis remains unclear. Grape seed proanthocyanidins extract
(GSPE) are one of the most widely distributed polyphenols with antioxidant properties. Here, we evaluated the mechanism
by which GSPE alleviates podocyte damage induced by high glucose and palmitic acid (HG/PA). Podocytes were cultured
in groups in normal environment; these were based on whether HG/PA and ML385 were present and the GSPE conc. 10, 25
and 50 mg/L. The flow cytometer, immunofluorescence staining, Western blot, quantitative real time-PCR (qRT-PCR), kit
method and fluorescence microscope were used, respectively. After HG/PA intervention, the podocyte morphology was
destroyed, the survival rate was reduced, the cell apoptosis was increased, and the fluorescence intensity of ROS detection
was increased, iron metabolism was disordered. After adding ML385, increased oxidative damage to podocyte, nuclear
factor erythroid 2-related factor 2 (Nrf2) and its downstream protein and mRNA expression levels were significantly
reduced. However, application of different concentrations of GSPE could reverse these situations. Our findings indicate that
GSPE regulates intracellular iron metabolism disorders and reduces the oxidative damage of podocytes possibly through
activation of the Nrf2 signaling pathway.
 
Date 2023-04-03T09:48:16Z
2023-04-03T09:48:16Z
2023-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61647
https://doi.org/10.56042/ijeb.v61i04.177
 
Language en
 
Source IJEB Vol.61(04) [Apr 2023]