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Differential activation of NOTCH1 pathway in HNSCC cell lines of different anatomical sites

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Title Differential activation of NOTCH1 pathway in HNSCC cell lines of different anatomical sites
 
Creator Mukhopadhyay, Debalina
Chakroborty, Balark
Basu, Mukta
Ghosh, Supratim
Kumar Panda, Chinmay
 
Subject Cancer stem cells
Cell cycle phase distribution
Head and neck squamous cell carcinoma
FaDu
γ-Secretase inhibitor
 
Description 244-251
Head and neck squamous cell carcinoma (HNSCC) is one of the most frequent types of cancers, and the role of
NOTCH1 pathway during development of HNSCC is debatable. Here, we have made an attempt to evaluate the NOTCH1
pathway status in HNSCC cell lines from different anatomical subsites. At first, mRNA expression status of NOTCH1
pathway associated genes (NOTCH1/JAG1/JAG2/HES1/HEY1/CD44/FBXW7/HIF1α/VEGF) was analysed in two HNSCC
cell lines: FaDu (hypopharyngeal carcinoma) and SCC9 (tongue carcinoma) and was compared with publicly available
database. Then, molecular profiling (RNA/protein) of the genes and cell cycle phase distribution analysis were done after
DAPT (γ-secretase inhibitor) administration at different concentrations on the cell lines to see the differential effect, if any.
High NOTCH1 pathway activation was noted in FaDu cell line than the SCC9. In cytotoxicity assay with DAPT, FaDu
showed more sensitivity than SCC9. Therefore, gradual decline of the expression of NOTCH1 pathway associated genes
was noted in FaDu with the increasing DAPT concentrations, leading to high S/G2-M arrest of the cell population.
Contrastingly, SCC9 showed significant reduced expression of the genes at higher concentration of DAPT with
comparatively low S/G2-M arrest of the cell population. The study demonstrates distinct NOTCH1 pathway signature in the
HNSCC cell lines of specific sub-sites of head and neck.
 
Date 2023-04-03T09:59:34Z
2023-04-03T09:59:34Z
2023-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61655
https://doi.org/10.56042/ijeb.v61i04.171
 
Language en
 
Source IJEB Vol.61(04) [Apr 2023]