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Susceptibility studies of farmed Pacific white Shrimp, Penaeus vannamei to non-AHPND strain of Vibrio parahaemolyticus

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Title Susceptibility studies of farmed Pacific white Shrimp, Penaeus vannamei to non-AHPND strain of Vibrio parahaemolyticus
 
Creator Ananda Raja, R., Sridhar, R., Balachandran, C., Palanisammi, A., Ramesh, S., Nagarajan, K
 
Subject AHPND, EMS, Penaeus vannamei, Vibrio parahaemolyticus
 
Description Not Available
Isolation and identification of bacteria from haemolymph, stomach and hepatopancreas
(HP) were carried out from 37 Penaeus vannamei farms in Tamil Nadu, India to find out
the prevalence of Vibrio parahaemolyticus (Vp) infections with special reference to acute
hepatopancreatic necrosis disease (AHPND). Bacterial identifications were done based on the
morphological, physiological and biochemical characteristics. Vp isolates were identified by
polymerase chain reaction (PCR) targeting Vp specific toxR and tlh genes, human pathogenic
tdh and trh genes, and AHPND causing AP1, AP2, AP3 (pirAvp) and AP4 (pirAvp and pirBvp)
genes. Histopathological and immunohistochemical (IHC) techniques were employed to identify
the pathology due to Vp in HP tissues. Based on the morphological, physiological, biochemical
and molecular characterization, 74 isolates were identified as Vp (35.14%), V. harveyi (21.62%),
V. anguillarum (16.22%), V. campbellii (10.81%), V. mimicus (8.11%), V. alginolyticus (5.41%),
and V. aeruginosa (2.7%). All isolates were negative for tdh, trh, AP1, AP2, AP3 and AP4 genes.
Histopathology and IHC revealed that the HP was the prime organ affected in Vp infection. It
further proved that the IHC could be used as an important tool to identify the establishment of
infection in hepatopancreatic tissues.
TANUVAS
 
Date 2023-02-13T06:38:09Z
2023-02-13T06:38:09Z
2022-01-01
 
Type Article
 
Identifier Not Available
2347-2774
2347-2774
http://krishi.icar.gov.in/jspui/handle/123456789/76090
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Veterinary and Animal Sciences Research