Record Details

Sequential analysis of erythrocyte aggregation in P. falciparum malaria with and without ASAQ therapy by optical signal and image analysis

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Sequential analysis of erythrocyte aggregation in P. falciparum malaria with and without ASAQ therapy by optical signal and image analysis
 
Creator Edison1, Maombi
Jeeva, JB
Singh, Megha
 
Subject Artemisinin combination therapy
LED Laser aggregometer
P. falciparum malaria
Parasitemia aggregation parameters
 
Description 331-338
The aggregation of erythrocytes is an important mechanism for blood flow through the cardiovascular system. In malaria,
this is complicated by infection caused by P. falciparum and is further complicated by the severity of parasitemia. Hence
analysis of this micro-mechanism is essential to know the changes in blood not only in diseased conditions but also after
artemisinin combination therapy (ASAQ) to alleviate suffering. For analysis purposes, aggregation of erythrocytes was
determined by LED laser aggregometer, represented in terms of various parameters related to the changes in laser transmitted
intensity. Formed aggregates are further analyzed by imaging and image-processing methods. For this study blood samples
from young adults (18 – 40 years old) infected with P. falciparum (n= 80), without any other serious illness, were performed.
These samples were selected based on the severity of parasitemia, and were divided into low (LP), medium1 (MP1), medium
2 (MP2), and high (HP) parasitemia. For three days, the selected individuals were treated with artemisinin-based combination
therapy ASAQ (Artesunate 4 mg/kg and amodiaquine 10 mg base/ kg once a day). Healthy subjects (n=20) without any history
of the disease were selected as a control group. The results, as obtained by various parameters, show a significant elevation of
aggregation of erythrocytes (P< 0.05) in P. falciparum malaria with the increase of parasitemia level. There was a decrease in
the aggregation after treatment on day four tending towards normal. Thus the current study shows the potential beneficial role of
ASAQ on erythrocytes aggregation, which may contribute to reducing the harmful effects on various organs in P. falciparuminfected
blood.
 
Date 2023-04-12T11:38:10Z
2023-04-12T11:38:10Z
2023-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/61726
https://doi.org/10.56042/ijbb.v60i4.64636
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(04) [April 2023]