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Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep in vitro

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Title Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep in vitro
Not Available
 
Creator Lalrinkima H
Jacob SS
Raina OK
Chandra D
Lalawmpuii K
Lalchhandama C
Behera P
Tolenkhomba TC
 
Subject ADCC
Cytotoxic killing
Fasciola gigantica NEJs
Superoxide dismutase
 
Description Not Available
Fasciola gigantica faces a series of threats from various free radicals produced by the host immune system during its invasion through the abdominal cavity and establishment in the bile duct of ruminants, limiting the fluke viability. The role of the superoxide radical produced by Muzaffarnagari sheep immune effector cells against F. gigantica newly excysted juveniles (NEJs) is highlighted in this study, as is the critical role of superoxide dismutase enzyme (SOD) in dismutation of superoxide radicals derived from host immune effector cells in vitro. Three concentrations of the ovine immune effector cells viz. 2.5, 5, and 10 × 106 cells were tested for their ability to induced cytotoxic killing of the parasite. All the three cell concentrations caused significant (p < 0.01) cytotoxic killing of NEJs in comparison to the control groups. Also, reduction of the immune effector cell concentration directly correlates with the NEJs killing. Attachment of immune effector cells to the parasite tegument in the presence of anti-F. gigantica antibodies was found to be critical in inducing NEJs killing via antibody-dependent cell-mediated cytotoxicity (ADCC). However, the addition of SOD greatly inhibits cytotoxic killing of NEJs, demonstrating the importance of SOD enzyme in fluke survival and parasite evasion of the host immunity. Thus, F. gigantica SOD warrants a promising candidate for immunoprophylactic studies in ruminants against the tropical liver fluke
Not Available
 
Date 2023-04-12T07:20:48Z
2023-04-12T07:20:48Z
2022-09-01
 
Type Research Paper
 
Identifier Lalrinkima H, Jacob SS, Raina OK, Chandra D, Lalawmpuii K, Lalchhandama C, Behera P, Tolenkhomba TC. Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep invitro. Exp Parasitol. 2022 Nov;242:108369. doi: 10.1016/j.exppara.2022.108369. Epub 2022 Sep 1. PMID: 36058254.
0014-4894
http://krishi.icar.gov.in/jspui/handle/123456789/76816
 
Language English
 
Relation Not Available;
 
Publisher Elsevier