Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep in vitro
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Title |
Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep in vitro
Not Available |
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Creator |
Lalrinkima H
Jacob SS Raina OK Chandra D Lalawmpuii K Lalchhandama C Behera P Tolenkhomba TC |
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Subject |
ADCC
Cytotoxic killing Fasciola gigantica NEJs Superoxide dismutase |
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Description |
Not Available
Fasciola gigantica faces a series of threats from various free radicals produced by the host immune system during its invasion through the abdominal cavity and establishment in the bile duct of ruminants, limiting the fluke viability. The role of the superoxide radical produced by Muzaffarnagari sheep immune effector cells against F. gigantica newly excysted juveniles (NEJs) is highlighted in this study, as is the critical role of superoxide dismutase enzyme (SOD) in dismutation of superoxide radicals derived from host immune effector cells in vitro. Three concentrations of the ovine immune effector cells viz. 2.5, 5, and 10 × 106 cells were tested for their ability to induced cytotoxic killing of the parasite. All the three cell concentrations caused significant (p < 0.01) cytotoxic killing of NEJs in comparison to the control groups. Also, reduction of the immune effector cell concentration directly correlates with the NEJs killing. Attachment of immune effector cells to the parasite tegument in the presence of anti-F. gigantica antibodies was found to be critical in inducing NEJs killing via antibody-dependent cell-mediated cytotoxicity (ADCC). However, the addition of SOD greatly inhibits cytotoxic killing of NEJs, demonstrating the importance of SOD enzyme in fluke survival and parasite evasion of the host immunity. Thus, F. gigantica SOD warrants a promising candidate for immunoprophylactic studies in ruminants against the tropical liver fluke Not Available |
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Date |
2023-04-12T07:20:48Z
2023-04-12T07:20:48Z 2022-09-01 |
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Type |
Research Paper
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Identifier |
Lalrinkima H, Jacob SS, Raina OK, Chandra D, Lalawmpuii K, Lalchhandama C, Behera P, Tolenkhomba TC. Superoxide dismutase inhibits cytotoxic killing of Fasciola gigantica newly excysted juveniles expressed by sheep invitro. Exp Parasitol. 2022 Nov;242:108369. doi: 10.1016/j.exppara.2022.108369. Epub 2022 Sep 1. PMID: 36058254.
0014-4894 http://krishi.icar.gov.in/jspui/handle/123456789/76816 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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