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Comparative whole genome analysis of Pasteurella multocida strains causing haemorrhagic scepticaemia (HS) in bovines

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Title Comparative whole genome analysis of Pasteurella multocida strains causing haemorrhagic scepticaemia (HS) in bovines
Not Available
 
Creator Prajapati A
Yogisharadhya R
Mohanty NN
Mendem SK
Nizamuddin A
Chanda MM
Shivachandra SB
 
Subject Bovines
Genome analysis
Haemorragic septicaemia
India
Pasteurella multocida
 
Description Not Available
Pasteurella multocida, a Gram-negative bacterium with ubiquitous nature, is known to affect wide range of host species worldwide with varied clinical manifestations including haemorrhagic septicaemia (HS) in bovines. Although, HS causing P. multocida strains were identified and characterized by conventional tools and PCR assays, diverse strains are indistinguishable by these tools in the face of disease outbreaks. In this study, draft genomes of three virulent P. multocida serotype B:2 strains (NIVEDIPm32, NIVEDIPm34 and NIVEDIPm35) were analyzed following whole genome sequencing, assembly, annotation and compared them with existing global genomes (n = 43) of bovine origin in the database. Three draft genomes of NIVEDIPm strains consisted of 40-52 contigs with GC content of ∼40.4%. The genome size and predicted genes content was ∼2.3 Mb and 2181-2189, respectively. Besides, the presence of various mobile genetic elements, antimicrobial resistance genes and biofilm related genes suggested their vital roles in virulence; further, adaptation to the host immune system as well as host pathogen interaction. Multi locus sequence analysis based on RIRDC scheme showed the presence of ST122 in all the three strains. wgMLST based phylogenic analysis suggested that HS causing Indian virulent field strains differed geographically and showed diversity from existing HS vaccine strain P52. The phylogenetic tree revealed that North Indian strains share high similarity with strains of Pakistan than South Indian Strain. Notably, a high divergence of SNPs between the HS causing circulating virulent strains of India and current HS vaccine strain P52 suggested an imminent need for relook in to HS vaccination strategy for livestock in India.
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Date 2023-04-12T07:18:49Z
2023-04-12T07:18:49Z
2022-03-24
 
Type Research Paper
 
Identifier Prajapati A, Yogisharadhya R, Mohanty NN, Mendem SK, Nizamuddin A, Chanda MM, Shivachandra SB. Comparative genome analysis of Pasteurella multocida serogroup B:2 strains causing haemorrhagic septicaemia (HS) in bovines. Gene. 2022 Jun 5;826:146452. doi: 10.1016/j.gene.2022.146452. Epub 2022 Mar 24. PMID: 35339640.
0378-1119
http://krishi.icar.gov.in/jspui/handle/123456789/76811
 
Language English
 
Relation Not Available;
 
Publisher Elsevier