Record Details

Silent Trypanosoma evansi infection in humans from India revealed by serological and molecular surveys, and characterized by variable surface glycoprotein gene sequences

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Silent Trypanosoma evansi infection in humans from India revealed by serological and molecular surveys, and characterized by variable surface glycoprotein gene sequences
Not Available
 
Creator Sengupta PP
Jacob SS
Chandu AGS
Das S
 
Subject Antibodies
Human
Silent infection
Trypanosoma evansi
Variable surface glycoprotein gene
 
Description Not Available
Abstract
Background
The importance of emerging atypical human trypanosomosis is gaining momentum due to increasing detection and its possible impact on human health. A cross sectional study of atypical human trypanosomosis due to Trypanosoma evansi was carried out in Kolkata and Canning area of West Bengal state of India where previously a death was reported.
Methods
In this study blood and serum samples from 173 individuals were collected during August to December 2014. To check the presence of antibodies against T. evansi, card agglutination test and for the presence of T. evansi specific DNA, PCR were conducted.
Results
T. evansi infection was identified in 5.2% (9/173) human blood samples by CATT serological test (Card agglutination test for trypanosomosis). PCR targeting VSG gene sequences suggested active T. evansi infection in 2.89% (5/173). VSG gene sequences herein determined for five isolates from human cases shared high similarity (89.4–100%). Phylogenetic inference clustered the human isolates with other isolates from different host species from India and other countries, forming a clade exclusive of Indian isolates (84.0 to 100% sequence similarity).
Conclusion
First report of symptomless human T. evansi infection detected by combined serological and PCR assays. First phylogenetic analysis of VSG gene sequences including human isolates of T. evansi in which Indian isolates of T. evansi from human and other hosts clustered in a single clade.
Not Available
 
Date 2023-04-12T07:18:24Z
2023-04-12T07:18:24Z
2022-02-12
 
Type Research Paper
 
Identifier Sengupta PP, Jacob SS, Chandu AGS, Das S. Silent Trypanosoma evansi infection in humans from India revealed by serological and molecular surveys, and characterized by variable surface glycoprotein gene sequences. Acta Trop. 2022 May;229:106369. doi: 10.1016/j.actatropica.2022.106369. Epub 2022 Feb 12. PMID: 35157840.
0001-706X
http://krishi.icar.gov.in/jspui/handle/123456789/76809
 
Language English
 
Relation Not Available;
 
Publisher Elsevier