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Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics

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Title Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics
Not Available
 
Creator Varughese HS
Chandrahasa C
Kavitha GM
Akhila DS
Gopinath S
Govindaiah K
Chandan T
Rao VCP
Hosamani M
Ramakrishnaiah S
Doddamane R
Hemadri D
Isloor S
 
Subject Rabies
nucleoprotein
cloning
in house immunodiagnostics
 
Description Not Available
Rabies viral nucleoprotein is proven to be antigenic and immunogenic in nature. Monoclonal antibody binding characterises the Rabies viral nucleoproteins immune reactivity. However, studies on reactivity of nucleoproteins to polyclonal antibodies are limited. In this study, a partial Rabies viral nucleoprotein gene of 516 bp was cloned into a pET 32a vector, and prokaryotic expression was induced to obtain a protein of 24.1 kDa. The protein obtained was further characterised by Western blot and indirect ELISA. Our research discovered Rabies nucleoprotein immune reactivity in polyclonal sera and suspected that the epitopes are conformational in nature. This study adds to the limited number of polyclonal sera based studies on Rabies nucleoprotein.
Not Available
 
Date 2023-04-13T06:33:52Z
2023-04-13T06:33:52Z
2022-12-12
 
Type Research Paper
 
Identifier Varughese HS, Chandrahasa C, Kavitha GM, Akhila DS, Gopinath S, Govindaiah K, Chandan T, Rao VCP, Hosamani M, Ramakrishnaiah S, Doddamane R, Hemadri D and Isloor S. (2022). Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics. The Pharma Innovation Journal, 12 516-519
2277-7695
http://krishi.icar.gov.in/jspui/handle/123456789/76849
 
Language English
 
Relation Not Available;
 
Publisher The Pharma Innovation