Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line
NOPR - NISCAIR Online Periodicals Repository
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Title |
Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line
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Creator |
Kumari, Snehal
Chaudhary, Neha Priya, Khushbu Rai, Geeta Gautam, Dev Nath Singh Singh, Narendra Kumar |
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Subject |
MTT assay
PBMCs cells SiHa Cells Teclenone β-sitosterol |
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Description |
182-188
Tephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from ethyl acetate extract of the seeds of T. purpurea (EETP). Quantity of TP-3 (6.05%) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5- di methyl thiazol -2-yl)-2,5-di phenyl tetrazolium bromide) assay. On the basis of spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and β-sitosterol respectively. Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC50 33.06 μgmL-1. TP-3 showed significant cytotoxicity at the concentration range from 20-50 μgmL-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 μgmL-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 μgmL-1 (cytotoxicity 24.19%). EETP showed significant cytotoxicity (IC50 value 113.63 μgmL-1) against SiHa cells at the concentration range from 75-150 μgmL-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 μgmL-1. |
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Date |
2023-04-13T12:25:49Z
2023-04-13T12:25:49Z 2023-04 |
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Type |
Article
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Identifier |
0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/61740 https://doi.org/10.56042/ijtk.v22i1.33137 |
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Language |
en
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Relation |
A61K 36/00
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Publisher |
NIScPR-CSIR, India
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Source |
IJTK Vol.22(1) [January 2023]
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