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Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line

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Title Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line
 
Creator Kumari, Snehal
Chaudhary, Neha
Priya, Khushbu
Rai, Geeta
Gautam, Dev Nath Singh
Singh, Narendra Kumar
 
Subject MTT assay
PBMCs cells
SiHa Cells
Teclenone
β-sitosterol
 
Description 182-188
Tephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from ethyl acetate extract of the seeds of T. purpurea (EETP). Quantity of TP-3 (6.05%) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5- di methyl thiazol -2-yl)-2,5-di phenyl tetrazolium bromide) assay. On the basis of spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and β-sitosterol respectively. Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC50 33.06 μgmL-1. TP-3 showed significant cytotoxicity at the concentration range from 20-50 μgmL-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 μgmL-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 μgmL-1 (cytotoxicity 24.19%). EETP showed significant cytotoxicity (IC50 value 113.63 μgmL-1) against SiHa cells at the concentration range from 75-150 μgmL-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 μgmL-1.
 
Date 2023-04-13T12:25:49Z
2023-04-13T12:25:49Z
2023-04
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/61740
https://doi.org/10.56042/ijtk.v22i1.33137
 
Language en
 
Relation A61K 36/00
 
Publisher NIScPR-CSIR, India
 
Source IJTK Vol.22(1) [January 2023]