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Self-incompatibility evidenced through scanning electron microscopy and pollination behaviour in Stevia rebaudiana

Indian Agricultural Research Journals

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Title Self-incompatibility evidenced through scanning electron microscopy and pollination behaviour in Stevia rebaudiana
 
Creator YADAV, ASHOK KUMAR
SINGH, S
RAJEEV, RAJEEV
 
Subject Floral biology, Pollination, Pollen dispersal, Scanning electron microscopy (SEM), Stevia rebaudiana
 
Description Floral development of stevia [Stevia rebaudiana (Bertoni)], as followed by light and scanning electron microscopy (SEM), was differentiated into 9 distinct stages, each having distinct landmark features. Tetrad formation takes place at stage-2 and microspore production starts. Highest pollen viability (72.8 per cent) was observed at stage 4, whereas, pollen grain production was recorded to be highest at stage-5. The stages-4 & 5 can be best utilized for pollen collection by dissecting out anthers for manual pollination as the pollen viability was recorded to be highest with maximum pollen production. These stages are important for pollen collection to achieve hybridization and get good seed set. Based on viable seed formation and per cent germination in different pollination treatments studied, pollinations using bumble bee thorax was most successful. No stevia pollen was observed to be dispersed at 2m away from the plant. The results indicate low pollen dispersal through air suggesting entomophilous pollination behavior of the crop. Whereas, no seed set was observed through selfing with bagging as well as selfing by hand-pollination. None of the self pollen adhering to the stigma was found germinated while emerging through anthers in a selfed flower. Although, a good seed set was observed in open pollination suggesting self-incompatibility in the crop.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-01-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37162
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 1 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37162/16601
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences