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Methanol extract of Pergularia daemia (Forssk.) Chiov. leaves induce apoptosis in triple-negative breast cancer through intrinsic pathway

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Title Methanol extract of Pergularia daemia (Forssk.) Chiov. leaves induce apoptosis in triple-negative breast cancer through intrinsic pathway
 
Creator GH, Akhil
Kariyil, Bibu John
Desai, Akshatha G.
John, Reni
SV, Vasudhar Bhat
 
Subject Anticancer
Cytotoxicity
DNA strand breaks
Hair knot plant
Lupeol
MDA-MB-231
Stinking swallowwort
Trellis-vine
Whitlow plant
 
Description 329-338
Most traditional anticancer medications are ineffective in treating triple negative breast cancer (TNBC), which has
enhanced resistance to chemotherapeutic agents. Herbs continue to be a key element in the discovery of anticancer
medications. In the present study, we assessed the cytotoxicity of methanol extract of leaves of Pergularia daemia (MLPD)
in MDA-MB-231 triple-negative breast cancer (TNBC) cell line. The cytotoxicity of MLPD was assessed using MTT assay
which revealed significant concentration-dependent cytotoxicity with an IC50 value of 35.95±3.57 μg/mL. Morphological
evaluation of MDA-MB-231 cells treated with MLPD showed cytotoxic changes like vacuole formation, altered
morphology, and reduction in the number of cells with apoptotic bodies formation. Staining techniques, such as acridine
orange and ethidium bromide (AO/EB) showed early apoptosis characterized by yellow-green fluorescence and crescentshaped
nucleus. Hoechst 33258 staining of MDA-MB-231 cells treated with MLPD showed nuclear marginalization
indicative of nuclear apoptotic changes. To assess the mitochondrial membrane potential (MMP), we did JC-1 staining and
the results revealed green fluorescence indicative of mitochondrial dependent apoptotic pathway. Comet assay confirmed
significant DNA damaging property in MDA-MB-231 cells treated with MLPD. Western blotting analysis showed
significant downregulation of Bcl-2 by 0.52±0.03 folds with no significant change in caspase-8 expression. Hence, the
present study demonstrated that MLPD possessed potent cytotoxic effect against MDA-MB-231 TNBC cells through
mitochondria mediated intrinsic pathway of apoptosis.
 
Date 2023-05-01T07:23:18Z
2023-05-01T07:23:18Z
2023-05
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/61829
https://doi.org/10.56042/ijeb.v61i05.850
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(05) [May 2023]