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Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs

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Title Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs
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Creator Lalzampuia H, Elango S, Biswal JK, Krishnaswamy N, Selvan RPT, Saravanan P, Mahadappa P, V Umapathi, Reddy GR, Bhanuprakash V, Sanyal A, Dechamma HJ
 
Subject FMDV 3A and 3B proteins · Deletion mutants · Infectivity
 
Description Not Available
The development of a negative marker vaccine against the foot-and-mouth disease virus (FMDV) will enhance the capabilities
to differentiate vaccinated from infected animals and move forward in the progressive control pathway for the control
of FMD. Here, we report the development of mutant FMDV of Asia1 with partial deletion of non-structural proteins 3A
and 3B and characterization of their infectivity and protection response in the guinea pig model. The deleted FMDV Asia1/
IND/63/1972 mutants, pAsiaΔ3A
and pAsiaΔ3A3B1
were constructed from the full-length infectious clone pAsiaWT,
the viable
virus was rescued, and the genetic stability of the mutants was confirmed by 20 monolayer passages in BHK21 cells. The
mutant Asia1 viruses showed comparable growth pattern and infectivity with that of AsiaWT
in the cell culture. However,
the AsiaΔ3A3B1
virus showed smaller plaque and lower virus titer with reduced infectivity in the suckling mice. In guinea
pigs, the AsiaΔ3A3B1
virus failed to induce the disease, whereas the AsiaΔ3A
virus induced typical secondary lesions of FMD.
Vaccination with inactivated Asia1 mutant viruses induced neutralizing antibody response that was significantly lower than
that of the parent virus on day 28 post-vaccination (dpv) in guinea pigs (P < 0.05). Furthermore, challenging the vaccinated
guinea pigs with the homologous vaccine strain of FMDV Asia1 conferred complete protection. It is concluded that the
mutant AsiaΔ3A3B1
virus has the potential to replace the wild-type virus for use as a negative marker vaccine after assessing
the vaccine worth attributes in suspension cell and protective efficacy study in cattle.
CAAST; ICAR-IVRI
 
Date 2023-05-02T05:43:03Z
2023-05-02T05:43:03Z
2022-01-01
 
Type Research Paper
 
Identifier Lalzampuia, H., Elango, S., Biswal, J.K. et al. Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs. Appl Microbiol Biotechnol 106, 273–286 (2022).
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http://krishi.icar.gov.in/jspui/handle/123456789/76915
 
Language English
 
Relation Not Available;
 
Publisher Springer