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Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard

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Title Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard
Not Available
 
Creator Behera SK
Sabarinath T
Ganesh B
Mishra PKK
Niloofa R
Senthilkumar K
Verma MR
Hota A
Chandrasekar S
Deneke Y
Kumar A
Nagarajan M
Das D
Khatua S
Sahu R
Ali SA
 
Subject dot ELISA dipstick test
latex agglutination test
leptospirosis
microscopic agglutination test
point-of-care
 
Description Not Available
Leptospirosis is a spirochaetal infection that possesses a broad host range affecting almost
all mammals. In the present study, the microscopic agglutination test (MAT) was compared with
recombinant LigA/B antigen-based point-of-care diagnostics such as the in-house IgM dot ELISA
dipstick test (IgM- DEDT) and the latex agglutination test (LAT) for the serodiagnosis of human
leptospirosis. The comparison of the MAT with these two point–of-care diagnostics was performed
using the MAT as the gold standard test and using Bayesian latent class modelling (BLCM), which
considers all diagnostic tests as imperfect. The N-terminal conserved region of the LigA/B protein
spanning the first to fifth big tandem repeat domains (rLigA/BCon1-5) was employed as a serodiag nostic marker in both of the bedside assays. A total of 340 serum samples collected from humans
involved in high risk occupations were screened using the MAT, IgM DEDT and LAT. During the early
phase of leptospirosis, BLCM analysis showed that the IgM DEDT and LAT had similar sensitivities
(99.6 (96.0–100)) and (99.5 (95.2–100)), respectively, while the single acute phase MAT had the lowest
sensitivity (83.3 (72.8–91.3)). Both the IgM DEDT and the LAT may be superior to the single acute phase MAT in terms of sensitivity during the early phase of infection and may be suitable for the
early diagnosis of leptospirosis. However, BLCM analysis revealed that the use of both acute and
convalescent samples substantially increased the sensitivity of the final MAT (98.2% (93.0–99.8%)) as
a test to diagnose human leptospirosis. Both the IgM DEDT and LAT can be employed as bedside
spot tests in remote locations where the MAT is not easily accessible
Not Available
 
Date 2023-05-02T06:01:14Z
2023-05-02T06:01:14Z
2022-06-13
 
Type Research Paper
 
Identifier Behera SK, Sabarinath T, Ganesh B, Mishra PKK, Niloofa R, Senthilkumar K, Verma MR, Hota A, Chandrasekar S, Deneke Y, Kumar A, Nagarajan M, Das D, Khatua S, Sahu R, Ali SA. Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard. Diagnostics. 2022; 12 (6): 1455.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/76932
 
Language English
 
Relation Not Available;
 
Publisher MDPI, Switzerland