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Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis

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Title Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis
Not Available
 
Creator Behera SK
Sabarinath T
Mishra PKK
Deneke Y
Kumar A
Chandrasekar S
Senthilkumar K
Verma M
Ganesh B
Gurav A
Hota A.
 
Subject bioinformatics tools
Dot ELISA dipstick test
in silico prediction
latex agglutination test
leptospirosis
 
Description Not Available
Leptospirosis is responsible for hampering the productivity of swine husbandry worldwide.
The aim of this study was to assess the efficacy of bioinformatics tools in predicting the three dimensional structure and immunogenicity of recombinant LigBCon1-5 (rLigBCon1-5) antigen.
A battery of bioinformatics tools such as I-TASSER, ProSA and SAVES v6.0 were used for the
prediction and assessment of the predicted structure of rLigBCon1-5 antigen. Bepipred-2.0, DiscoTope
v2.0 and ElliPro servers were used to predict linear and conformational epitopes while T-cell epitopes
were predicted using NetMHCpan 4.1 and IEDB recommended 2.22 method for MHC Class I and
II peptides respectively. The results obtained using various in silico methods were then compared
with wet lab experiments comprising of both primary (IgG Dot ELISA Dipstick test) and secondary binding assays (Latex Agglutination Test [LAT]) to screen 1153 porcine serum samples. The three dimensional structure of rLigA/BCon1-5 protein as predicted by I-TASSER was found to be reliable
by Ramachandran Plot and ProSA. The ElliPro server suggested 10 and three potential linear and
conformational B-cell-epitopes, respectively, on the peptide backbone of the rLigA/BCon1-5 protein.
The DiscoTope prediction server suggested 47 amino acid residues to be part of B-cell antigen.
Ten of the most efficient peptides for MHC-I and II grooves were predicted by NetMHCpan 4.1 and
IEDB recommended 2.22 method, respectively. Of these, three peptides can serve dual functions
as it can fit both MHC I and II grooves, thereby eliciting both humoral-and cell-mediated immune
responses. The prediction of these computational approaches proved to be reliable since rLigBCon1-5
antigen-based IgG Dot ELISA Dipstick test and LAT gave results in concordance to gold standard
test, the Microscopic Agglutination Test (MAT), for serodiagnosis of leptospirosis. Both the IgG Dot ELISA Dipstick test and LAT were serodiagnostic assays ideally suited for peripheral level of animal
health care system as “point of care” tests for the detection of porcine leptospirosis.
Not Available
 
Date 2023-05-02T06:05:08Z
2023-05-02T06:05:08Z
2021-08-26
 
Type Research Paper
 
Identifier  Behera SK, Sabarinath T, Mishra PKK, Deneke Y, Kumar A, Chandrasekar S, Senthilkumar K, Verma M, Ganesh B, Gurav A, Hota A. Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis. Pathogens. 2021; 10 (9): 1082
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/76940
 
Language English
 
Relation Not Available;
 
Publisher MDPI, Switzerland