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Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis

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Title Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis
Not Available
 
Creator Behera SK
Sabarinath T
Deneke Y
Bansal SK
Mahendran K
Kumar A
Senthil K
Verma MR
Chandrasekar S
Atif Ali S.
 
Subject DIVA based test
ELISA
Latex agglutination test
Canine leptospirosis
Spatial variation
 
Description Not Available
Background: Canine leptospirosis is a serious public health concern. Aims: This study aims to investigate the feasibility of
conserved first to fifth domains of recombinant Leptospira immunoglobulin like protein B antigen (rLigBCon1-5) as a serodiagnostic
marker for detecting canine leptospirosis. Methods: A total of 340 unvaccinated canine serum samples were screened using
microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent
assay (I-ELISA). Further, 60 vaccinated canine sera were screened using MAT and rLigBCon1-5 based latex agglutination test
(LAT). Results: Microscopic agglutination test results revealed seropositivity of 28.6%. The relative sensitivity, specificity, and
accuracy of IgG I-ELISA in comparison to MAT were 100%, 96.0%, and 97.2%, respectively. Out of 60 vaccinated sera, 46 sera
reacted with MAT alone, and eight sera reacted by both tests, while six sera were non-reactive with both tests. Anti-LigB antibodies
were detected in eight canine sera by rLigBCon1-5 based LAT. In five LAT reactive sera, agglutinins of locally circulating
Leptospira serovars Grippotyphosa (n=4) and Australis (n=1) were detected. In three LAT reactive sera, agglutinins against
Icterohaemorrhagiae (n=3) produced due to natural infection were present. Conclusion: Immunoglobulin G based indirect ELISA
assay (IgG I-ELISA) can be employed as an alternative test instead of MAT. rLigBCon1-5 based LAT detected anti-LigB antibodies
in eight vaccinated sera where the vaccine failure occurred partially or totally due to the limited efficacy spectrum of Nobivac® RL
and cold chain breakage. This vaccine could not provide cross-protection against locally circulating Leptospira serovars. The
recombinant LigBCon1-5 antigen based LAT possesses capability of differentiating infected from vaccinated individuals (DIVA
capability) when employed as a pen-side test for detecting canine leptospirosis.
Not Available
 
Date 2023-05-02T06:13:27Z
2023-05-02T06:13:27Z
2021-02-28
 
Type Research Paper
 
Identifier  Behera SK, Sabarinath T, Deneke Y, Bansal SK, Mahendran K, Kumar A, Senthil K, Verma MR, Chandrasekar S, Atif Ali S. Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis. Iran. J. Vet. Res. 2021; 22(2): 120–128
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/76960
 
Language English
 
Relation Not Available;
 
Publisher Shiraz University