Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis
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Title |
Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis
Not Available |
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Creator |
Behera SK
Sabarinath T Deneke Y Bansal SK Mahendran K Kumar A Senthil K Verma MR Chandrasekar S Atif Ali S. |
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Subject |
DIVA based test
ELISA Latex agglutination test Canine leptospirosis Spatial variation |
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Description |
Not Available
Background: Canine leptospirosis is a serious public health concern. Aims: This study aims to investigate the feasibility of conserved first to fifth domains of recombinant Leptospira immunoglobulin like protein B antigen (rLigBCon1-5) as a serodiagnostic marker for detecting canine leptospirosis. Methods: A total of 340 unvaccinated canine serum samples were screened using microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent assay (I-ELISA). Further, 60 vaccinated canine sera were screened using MAT and rLigBCon1-5 based latex agglutination test (LAT). Results: Microscopic agglutination test results revealed seropositivity of 28.6%. The relative sensitivity, specificity, and accuracy of IgG I-ELISA in comparison to MAT were 100%, 96.0%, and 97.2%, respectively. Out of 60 vaccinated sera, 46 sera reacted with MAT alone, and eight sera reacted by both tests, while six sera were non-reactive with both tests. Anti-LigB antibodies were detected in eight canine sera by rLigBCon1-5 based LAT. In five LAT reactive sera, agglutinins of locally circulating Leptospira serovars Grippotyphosa (n=4) and Australis (n=1) were detected. In three LAT reactive sera, agglutinins against Icterohaemorrhagiae (n=3) produced due to natural infection were present. Conclusion: Immunoglobulin G based indirect ELISA assay (IgG I-ELISA) can be employed as an alternative test instead of MAT. rLigBCon1-5 based LAT detected anti-LigB antibodies in eight vaccinated sera where the vaccine failure occurred partially or totally due to the limited efficacy spectrum of Nobivac® RL and cold chain breakage. This vaccine could not provide cross-protection against locally circulating Leptospira serovars. The recombinant LigBCon1-5 antigen based LAT possesses capability of differentiating infected from vaccinated individuals (DIVA capability) when employed as a pen-side test for detecting canine leptospirosis. Not Available |
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Date |
2023-05-02T06:13:27Z
2023-05-02T06:13:27Z 2021-02-28 |
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Type |
Research Paper
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Identifier |
Behera SK, Sabarinath T, Deneke Y, Bansal SK, Mahendran K, Kumar A, Senthil K, Verma MR, Chandrasekar S, Atif Ali S. Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of Leptospira interrogans serovar Pomona in canine leptospirosis. Iran. J. Vet. Res. 2021; 22(2): 120–128
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/76960 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Shiraz University
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