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Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity

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Title Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity
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Creator Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham G J, Curry S, Lomonossoff G P, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart D I, Charleston B, Jones I M
 
Subject Efficient expression of FMDV empty capsids in insect cells after moderation of 3C protease action, Expression cassette productive in multiple insect cell lines.,Empty capsids visualised by transmission electron microscopy, Empty capsids react with wide range of positive sera as well as authentic virus, Efficient empty capsid synthesis may allow development as a vaccine.
 
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Foot-and-mouth disease virus (FMDV) is a significant economically and distributed globally pathogen of Artiodactyla. Current vaccines are chemically inactivated whole virus particles that require large-scale virus growth in strict bio-containment with the associated risks of accidental release or incomplete inactivation. Non-infectious empty capsids are structural mimics of authentic particles with no associated risk and constitute an alternate vaccine candidate. Capsids self-assemble from the processed virus structural proteins, VP0, VP3 and VP1, which are released from the structural protein precursor P1-2A by the action of the virus-encoded 3C protease. To date recombinant empty capsid assembly has been limited by poor expression levels, restricting the development of empty capsids as a viable vaccine. Here expression of the FMDV structural protein precursor P1-2A in insect cells is shown to be efficient but linkage of the cognate 3C protease to the C-terminus reduces expression significantly. Inactivation of the 3C enzyme in a P1-2A-3C cassette allows expression and intermediate levels of 3C activity resulted in efficient processing of the P1-2A precursor into the structural proteins which assembled into empty capsids. Expression was independent of the insect host cell background and leads to capsids that are recognised as authentic by a range of anti-FMDV bovine sera suggesting their feasibility as an alternate vaccine.
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Date 2023-05-02T04:41:45Z
2023-05-02T04:41:45Z
2013-02-01
 
Type Research Paper
 
Identifier Claudine Porta, Xiaodong Xu, Silvia Loureiro, Saravanan Paramasivam, Junyuan Ren, Tara Al-Khalil, Alison Burman, Terry Jackson, Graham J. Belsham, Stephen Curry, George P. Lomonossoff, Satya Parida, David Paton, Yanmin Li, Ginette Wilsden, Nigel Ferris, Ray Owens, Abhay Kotecha, Elizabeth Fry, David I. Stuart, Bryan Charleston, Ian M. Jones, Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity, Journal of Virological Methods, Volume 187, Issue 2, 2013, Pages 406-412, ISSN 0166-0934
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http://krishi.icar.gov.in/jspui/handle/123456789/76894
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER